Comparison of In Vitro Culture Immersion System and The Effect of Immersion Variation on Chili Pepper Embryo Cultures (Capsicum annuum L. var annuum) Growth in TIS RITA Bioreactor

• Main Author: Ishaq Agasi, Harryyanto
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/43132
• Institution: Institut Teknologi Bandung

Indonesia’s cayenne pepper consumption increased from 1,26 to 1,89 kg per capita each year in 2016-2018. Therefore, it needs to be balanced with an efficient and sustainable cayenne pepper production system. Double haploid technology is a technology that able to produce a genetically homozygous plant with its mother cell. Commonly, the current embryo maturation protocol uses agar medium, while theoriticaly, nutrition on the liquid medium is easier to be absorbed, and so cultivating cayenne pepper embrios on a liquid medium becomes a challenge. However, fully immersed in-vitro cultivation in the liquid medium could evoke a new problem, which is vitrification, therefore this research used the temporary immersion system of TIS RITA bioreactor. The objective of this research was to compare growth performance of cayenne pepper embryos (Capsicum annuum L. var annum) which were cultivated in bilayer medium, fully immersed liquid medium, and in temporary immersion system bioreactor (TIS RITA) liquid medium with immersing variations of 1 minute in every 4 hours (4H) and 1 minute in every 8 hours (8H). Embryos were cultivated under the white light for 7 hours, red-blue LED (4:1) for 5 hours, and in the dark for 12 hours. Measured parameters were fresh weight, dry weight, sucrose concentration, conductivity, pH, and medium viscosity of TIS RITA. The result of a comparison between the full and temporary immersion system showed that the growth performance of the embryo in the temporary immersion system was better in increasing its biomass than the full immersion system with the percentage of fresh weight and dry weight gain were 39% and 25% for TIS RITA 4H, while on TIS RITA 8H were 34% and 36%. The variation of immersion time in TIS RITA did not produce any significant difference on the growth of embryo.