USE OF CONSORTIUM BACTERIA Halomonas aquamarina, Halomonas alkaliphila, Bacillus cereus, MICROALGAE Chaetoceros sp., AND NITRIFICATION BACTERIA TO IMPROVE GROWTH PERFORMANCE AND WHITE SHRIMP’S RESISTANCE (Litopenaeus vannamei) TO Vibrio sp.

• Main Author: Diffa Aulia Qatrunnada, Igretia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/43133
• Institution: Institut Teknologi Bandung

Vibriosis is a major problem facing the white shrimp aquaculture industry (Litopenaeus vannamei) because it can cause slow growth, even death. Vibriosis is caused one of them by Vibrio harveyi bacteria that has reached the quorum (106 CFU / ml). Since the use of antibiotics is prohibited because of the impact of resistance caused, the use of probiotic microbes that are able to control Vibrio sp. This study aims to determine the effect of the addition of a consortium of probiotic bacteria Halomonas aquamarina, H. alkaliphila, Bacillus cereus, microalgae Chaetoceros sp., and nitrifying bacteria on the biological parameters of white shrimp to control Vibrio sp. This study was divided into 2 stages, namely the challenge test of the addition of a bacterial consortium and V. harveyi with lethal concentrations (103 CFU / ml) on white shrimp larvae (density of 65 animals / liter) and the addition of a consortium of probiotic bacteria and V. harveyi with nonlethal concentrations ( 103CFU / ml) on enlargement of white shrimp (density of 500 individuals /m3). This study aims to: 1) determine the survival rate of the after challenge test on white shrimp larvae with 6 variations of treatment: control without the addition of a consortium of probiotic bacteria and V. harveyi (P1), positive control of the addition of a bacterial consortium (P2), negative control addition of V. harveyi (P3), the addition of a bacterial consortium together with V. harveyi (P4), the addition of a bacterial consortium 24 hours before V. harveyi (P5), and the addition of a bacterial consortium 24 hours after V. harveyi (P6)., 2) determine growth performance (survival, average weight, Feed Conversion Ratio) on the enlargement of white shrimp with variations in treatment: positive control of the addition of a consortium of bacteria (A), addition of consortium bacteria 24 hours before V. harveyi (B), negative control of the addition of V. harveyi bacteria (C), and control without the addition of a consortium of probiotic bacteria and V. harveyii (D). The results shown in the challenge test were the P5 treatment (50.00 ± 1.82%) had the highest survival rate which was significantly different (p <0.05) with P4 (43.03 ± 1.07%) and negative control P3 ( 42.26 ± 2.32%), but not significantly different from P6 (46.46 ± 2.32%). At enlargement, the survival of all treatments did not differ significantly, the average weight of shrimp A (4.30 ± 2.20 g), B (3.57 ± 1.39 g), and D (3.73 ± 1.46 g ) not significantly different, but significantly different (p <0.05) with negative control C (0.73 ± 0.20 g). The lowest Feed Conversion Ratio (FCR) is owned by B (1.19 ± 0.11), followed by A (1.76 ± 0.30), D (2.05 ± 0.22), and C (2.59 ± 1.98) and there were no significant differences between all treatments. It can be concluded that the addition of a consortium of probiotic bacteria earlier than V. harveyi in the challenge test with lethal concentration can increase survival in shrimp larvae. In contrast to the challenge test, the addition of probiotic bacteria earlier than V. harveyi on enlargement with nonlethal concentration did not affect survival, but could increase the average weight of shrimp produced with the best FCR value.