AGARWOOD ARTIFICIAL PRODUCTION IN Gyrinops versteegii (Gilg.) Domke INOCULATED BY Fusarium solani
Agarwood is a resinous wood containing secondary metabolites produced by Thymelaeaceae. Production of agarwood in their natural habitat could takes up to a hundred years. Therefore, various induction techniques have been explored to artificially induce agarwood in cultivated plants and to shorten it...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/43160 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Agarwood is a resinous wood containing secondary metabolites produced by Thymelaeaceae. Production of agarwood in their natural habitat could takes up to a hundred years. Therefore, various induction techniques have been explored to artificially induce agarwood in cultivated plants and to shorten its process, including induction by fungal inoculation. In this study, one of the most abundant agarwood-producing trees in Indonesia, namely Gyrinops versteegii was injected and inoculated with two different strains of endophytic fungi Fusarium solani isolated from Gorontalo (GSL1) and Jambi (GSL2) Provinces. Wounded plant without fungal inoculation (PDB medium only) and healthy plant without wounding were used as control treatment. The analysis were done for three and six months after inoculation. The results revealed that the inoculated G. verstegii generates extensive resinous zone on the wood tissues since three months of inoculation. Microscopic analysis showed that agarwood resin was accumulated in interxylary phloem (IP) structure on secondary xylem. Olfactory test showed that three months inoculation samples has no significant difference with six months inoculation sample, but GSL1 and GSL2 sample were significantly different with wounding without fungal inoculation. Gas chromatography (GC-MS) analysis revealed that fatty acids were dominantly detected in ethyl acetate extract, meanwhile chromones and sesquiterpenes were detected in methanol extract. Several chromone such as 2-(2-phenylethyl)chromen-4-one, 6-methoxy-2-(2-phenylethyl)chromen-4-one dan 6,7-dimethoxy-2-(2-phenylethyl)chromen-4-one were only detected in the fungal treated samples. Alloaromadendrene, beta-Eudesmol and beta-Selinene from sesquiterpenes also only found in fungal treated samples. Taken together, this technique would be beneficial to stimulate it rapid accumulation in cultivated agarwood producing trees.
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