The Effect of Incubation Time and Enzymes-Substrate Ratio to the IgG Antivenom Cleavage with a Mathematical Modelling of Enzymatic Reaction Kinetics

• Main Author: Ayu Lestari, Nadya
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/43280
• Institution: Institut Teknologi Bandung

Enzymatic reaction process with pepsin commonly used for clapping IgG molecule into F(ab’)2 fragment. That product will be used for creating serum that works effectively and decrease the side effect that may be occurred in the neutralization process. Mathematical approaching is used to optimize reaction by predicting Michaelis-Menten catalytic coefficient and maximum velocity. Independent variable that’s used in this research is the time of incubation and ratio between enzyme and substrate. This mathematical method basically uses Michaelis-Menten kinetics reaction equation for finding parameter with MATLAB software. Mathematical modelling will be created by using that parameter to become a differential equation. That model will be compared with model which has been created by parameter from curve fitting method. All of modelling is done by Berkeley Madonna software that integrated with Microsoft Excel. The result, parameter that come from Michaelis-Menten approaching could not fit with researching’s data well. In spite of that, it can give information that velocity of the reaction is decreasing over time and it ends in 125 minutes, the other hands the optimum ratio of enzyme substrate is between 1:80 and 1:400. From Michaelis-Menten approaching found that Vmax 4,21x10-3 /minute and Km 3,93x10-4, meanwhile curve fitting method gives bigger number that Vmax 0,015/minute. The final modelling is using parameter based on substrate that is 0,079 mg/ml for K[S] and 0,0326 /minute and this model is the most fit than the others.