Protein And Phycocyanin Production From Spirulina (Arthrospira platensis) Using Anaerobically Digested Dairy Manure Wastewater (ADDMW) As A Substrate With Addition Of Salinity Treatment

• Main Author: Rosanti, Serafina
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/43282
• Institution: Institut Teknologi Bandung

Spirulina (Arthospira platensis) is one of the most known food supplements, commonly called as “superfood” due to its high protein content and properties and efficacy to the human body. Unfortunately, Spirulina production is still constrained due to its high cost of cultivation medium and unoptimized cultivation system. We examined the use of anaerobically digested dairy manure wastewater (ADDMW) as a low-cost alternative cultivation medium for Spirulina. In addition, we add salinity to the medium to increase the production. The salinity treatment used in this research was 15 ppt, 25 ppt, and 35 ppt with 0 ppt as the control. Cultivation of A. platensis was carried out for 14 days using 1 litre capacity culture bottles. The best salinity variation for biomass yield and productivity was 35 ppt (37.40 g of biomass/g substrate and 0.0537 g/day). The optimum salinity for producing protein and phycocyanin from A. platensis using ADDMW medium is 35 ppt and 25 ppt respectively with yield of protein reached 3,144 g protein/g substrate and yield of phycocyanin reached 30,79 g phycocyanin/g substrate. Beside producing protein and phycocyanin, the growth of A. platensis in ADDMW could also be used as a phycoremediation process for orthophosphate, ammonium and nitrate in the medium. Orthophosphate removal efficiency is directly proportional to medium salinity however, it does not give a significant difference on ammonium removal efficiency. Nitrate removal efficiency is affected by the medium’s salinity, although it was disturbed by nitrification process that occurred during day 12-14.