KAJIAN PENGARUH DIDODESILDIMETILAMONIUM BROMIDA PADA NANOEMULSI KURKUMIN DAN UJI ANTIVIRUSNYA PADA SEL A549 YANG DIINFEKSI OLEH VIRUS DENGUE SEROTIPE 1 DAN 2

• Main Author: Riqqah Hassan, Siti
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/43948
• Institution: Institut Teknologi Bandung

Curcumin is a compound isolated from the rhizome of Curcuma domestica L. (turmeric) that shows many potentials, one of them is antiviral property. Despite the promising potentials, curcuminbased drug development is hindered due to its poor solubility and cell uptake, and easily degraded by various factors. This study aimed to develop a nanoemulsion containing curcumin (nanocurcumin) with the surface charge that was different from the surface charge of the previous formulation. Cationic nanocurcumin was formed by adding a DDAB compound (didodecyldimethylammonium bromide) into the nanoemulsion system. The addition of DDAB was aimed to change the surface charge of the nanocurcumin to be positive and to increase the cellular uptake of curcumin. Physical characteristics of both nanocurcumin and cationic nanocurcumin were evaluated using particle analyzer to measure particle size, polydispersity index, and zeta potential; transmission electron microscope to observe morphology of nanocurcumins; UV-Vis spectrophotometer to determine loading capacity of curcumin in both nanoemulsion systems. Evaluation of cell cytotoxicity potential was carried out through CC50 values using MTT assay method using a microplate reader at a wavelength of 570 nm to measure the number of viable cells, while the evaluation of antiviral potential was carried out by determining the IC50 value under cotreatment condition using a two steps RT-qPCR to measure the concentration of viral RNA. Nanocurcumin and cationic nanocurcumin had the sizes of 24,8 ± 2,5 nm and 28,17 ± 4,02 nm; polydispersity indexs of 0,189 ± 0,385 and 0,447 ± 0,027; zeta potentials of -4,3 ± 0,5 mV and +2,2 ± 0,17 mV; spheric and uniform in size with the size of cationic nanocurcumin slightly larger than nanocurcumin; loading capacities of 93,75 ± 0.95 % and 99 ± 0.50 % respectively. The CC50 value obtained was 21,42 ?g/mL for nanocurcumin and 2,1 ?g/mL for cationic nanocurcumin. The IC50 obtained was 1,688 ?g/mL for nanocurcumin and 0,777 ?g/mL for cationic nanocurcumin against DENV-1, while the IC50 value was recorded as 4,809 ?g/mL for nanocurcumin and 1,764 ?g/mL for cationic nanocurcumin against DENV-2. Based on these results, cationic nanocurcumin was more cytotoxic against A549 cells and was more virucidal against dengue virus than nanocurcumin, but further testing was needed to support that conclusion.