FORMULATION FREEZE DRIED OF SURFACE MODIFIED CHITOSAN-ACEMANNAN LIPID NANOPARTICLES AS RIFAMPICIN DELIVERY SYSTEM
Rifampicin is one of the antibiotics used to eliminate intracellular infections Staphylococcus aureus (SA). This research purposed to formulate rifampicin using lipid nanoparticles to improve its stability and intracellular accumulation. The nanoparticles were surface modified using chitosan-acem...
Saved in:
| Main Author: | |
|---|---|
| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/44087 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Rifampicin is one of the antibiotics used to eliminate intracellular infections
Staphylococcus aureus (SA). This research purposed to formulate rifampicin
using lipid nanoparticles to improve its stability and intracellular accumulation.
The nanoparticles were surface modified using chitosan-acemannan to facilitate
cellular internalization. Rifampicin nanoparticles had a size of 641.7 ± 48.75 nm
with polydispersity index of 0.33 ± 0.058 as well as zeta potential -5.34 ± 0.38.
Lyophilized rifampicin nanoparticles with the addition of cryoprotectant of 2,5%
mannitol showed the best particle growth inhibition. The qualitative analysis
testing showed increasing localization chitosan-acemannan modified
nanostructured lipid carrier (NLC) compared to chitosan and unmodified NLC in
BALB 3T3 and Vero Cells. The amount of rifampicin accumulation within BALB
3T3 and Vero cells was higher compared to NIH 3T3 cells. The minimum
inhibitory and bactericidal inhibitions of nanoparticles and solution were similar
which showed maintaining activity rifampicin during encapsulation. Rifampicin
nanoparticles showed antibacterial activity higher than rifampicin solution
against intracellular Staphylococcus aureus.
|
|---|