UJI AKTIVITAS ANTIJAMUR DAUN AWAR-AWAR (Ficus septica Burm.f.) TERHADAP Candida albicans DAN ISOLASI SENYAWA AKTIF ANTIJAMUR

• Main Author: Christina, Maria
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44120
• Institution: Institut Teknologi Bandung

Awar-awar (Ficus septica Burm.f.) is a widely distributed plant in Java. Awar-awar leaves contain secondary metabolites such as flavonoids, phenols, tannins and saponins. This compound has pharmacological activity as an antimicrobial. Candida albicans is a common commensal organism in the intestine, mouth and vagina. This fungus causes some broad spectrum of diseases such as mouth ulcers, vaginitis, endocarditis and septicemia. This study aims to isolate antifungal active compounds from awar-awar leaves against Candida albicans by diffusion agar method. The crude drug powder of awar-awar leaf was extracted by reflux method using ethanol and concentrated. The ethanol extract was then fractionated by liquid-liquid extraction using solvents with increased polarity, namely nhexane and ethyl acetate then the fractions were monitored using thin layer chromatography (TLC). The fraction that has the highest activity was subfractionated and determined the antifungal acitivity. The subfraction with the largest inhibitory diameter was purified by preparative TLC. The isolate was tested for its purity by a single development TLC method with three different mobile phases and two dimensions TLC to obtain pure isolate. The isolate was tested for antifungal activity by diffusion agar and was characterized using specific spray reagent and TLC-spectrophotodensitometry. The results showed that ethanol extract gave activity at 30% concentration of 10.60 ± 1.64 mm. The highest antifungal activity was found in the ethyl acetate fraction with a inhibiting diameter of 9.60 mm. Subfaction A from the results of subfactionation of ethyl acetate fraction had the highest antifungal activity with inhibition diameter of 8.27 ± 1.30 mm. Pure isolate have antifungal activity of 8.2 mm. Isolate A gave positive result with specific citroborate and FeCl3 spray reagent. The isolate was predicted to be a flavonoid compound subgroup isoflavones with two peaks at maximum wavelengths of 253 and 314 nm.