EFFECT OF LEAD NITRATE ON THE EMBRYONIC DEVELOPMENT OF THE DOMESTIC FOWL (GALLUS DOMESTICUS) INDIAN RIVER (IR) STRAIN

• Main Author: Pabittei A. Solulipu<br>NIM. S2 814109, Halifah
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/4423
• Institution: Institut Teknologi Bandung

</i><b>Abstract: </b><p align=\"justify\"><i> The effect of lead nitrate on the embryonic development of the domestic fowl (<u>Gallus domesticus</u>) Indian River (IR) strain has been investigated, by injecting the substance at the dose levels of 0,02 mg, 0,002 mg, 0,0004 mg and 0,0002 mg/egg, into the yolk sac of 4-day old embryos. The embryos were examined on day 16 of incubation. It was found that the mortality of the experimental embryos of all doses were significantly higher (p<0,01) than those of the controls, while its mean body weight were significantly lighter (p<0,05 and p<0,01). The significant occurance of the external malformations were obtained in the brain, eyes, feathers, feet and toes (p‹0,05 and p<0,01) of the experimental embryos. Hemorrhage and edema were also found. The histological lesions were observed in the liver, kidney and gonad, included : blood congestion in the blood vessels and sinusoids of the liver, dissociation of the hepatic cords, degeneration and vacuolization of the liver cells, degeneration of the renal tubules and the increasing number of the connective tissue among the seminiferous tubules of the testis and in the cortex of the ovary. Skeletal defects were found on the jaws, ribs, radius and tibiotarsus bones. A retarded ossification was noted inhe supraoccipital and parietal bones of the skull, in the sacral and caudal vertebrae and in the phalanx of the legs of the experimental embryos. The accumulation of lead in the embryonic tissues were detected by the Atomic Absorption Spectroscopy (AAS) method and it was shown that the highest accumulation was found in the bone, then in the liver and the lowest was found in the brain. It is concluded that lead nitrate is toxic and teratogenic to the embryonic development of the domestic fowl IR. strain.</p>