PENGEMBANGAN NANOPARTIKEL EMAS-KURKUMIN: KAJIAN IN VITRO DAN IN OVO

• Main Author: Mulya, Ardyanto
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44266
• Institution: Institut Teknologi Bandung

Curcumin 1,7-bis (4-hydroxy-3-methoxyphenol)-1, 6-heptadiene-3, 5-dione is the main active component of Curcuma longa L. Curcumin has been known possessing diverse pharmacological effects as therapeutic agent, one of them as anti fibrosis. However, the clinical efficacy of curcumin is limited due to its low dissolution, absorption, and extensive metabolism in the liver to inactive compounds after oral administration. Therefore, one of the promising strategy is applying nanotechnology to enhance bioavailability of lipophilic drugs such as curcumin. We developed curcumin-gold nanoparticles using a simple synthesis method via mild condition of chemical reaction. The formation of gold nanoparticle can be used as a carrier for curcumin for specific delivery into the liver. Curcumin-gold nanoparticles were synthesized by conjugating curcumin in various pHs and concentrations with HAuCl4 1 mM solution. Successful conjugation was indicated by color alteration from pale yellow to burgundy red. Curcumin-gold nanoparticle was characterized by UV-visible spectrophotometry, infrared spectrophotometry, differential scanning calorimetry, X-Ray diffraction, particle size, polidispersity index and zeta potential. The stability of curcumin-gold nanoparticle was performed in vitro using rat liver homogenate and compared with free curcumin. Curcumin-gold nanoparticle and free curcumin were measured at 0, 30, 60 and 120 minutes after incubation. Curcumin level was analyzed using High Performance Liquid Chromatography (HPLC). The early safety study was also performed in ovo to monitore the growth of chicken embryo. The optimum pH of curcumin solution to form curcumin-gold nanoparticle was 9.3 with molar ratio of curcumin to HAuCl4 1:1. Based on result of characterization, partial interaction between curcumin and HAuCl4 was detected. In the stability assay using liver homogenate, it is shown that the decreased of curcumin gold nanoparticle from 0 to 120 minutes was 19,705%, while free curcumin was 53,804%. In the in ovo study, it is shown that development of chicken embyros was not influenced by the presence of curcumin gold nanoparticle 300 ppm and no difference phenomena was observed when compared with untreated group.