PENGEMBANGAN FORMULA MATRIKS BERBASIS MIKROPARTIKEL KITOSAN SEBAGAI SISTEM PENGHANTARAN EKSTRAK ETANOL DAUN BINAHONG UNTUK KULTIVASI SEL FIBROBLAS
The important skin wound healing process, including fibroblast proliferation and collagen formation, can be facilitated by an adequate biodegradable matrix. The aim of this research is to develope a chitosan-based wound dressing formula containing microparticles as delivery system for ethanolic b...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/44267 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The important skin wound healing process, including fibroblast proliferation and collagen
formation, can be facilitated by an adequate biodegradable matrix. The aim of this research is to
develope a chitosan-based wound dressing formula containing microparticles as delivery system
for ethanolic binahong extract for in vitro collagen production testing. Binahong extract can
increase the production of collagen in fibroblast cell. Aloe vera was added to the matrix for inducing
the adhesion and proliferation of cells in the early step. The concentrations of extract and Aloe vera
were optimized for cell viability and collagen content. Microparticles were prepared by double
emulsion-solvent evaporation and solidified using sodium tripolyphosphate (STPP) or lecithin as
cross-linking agent. Various biodegradable polymers such as gelatin, PVA, and chitosan were
selected to produce 2-week-degraded matrix and the highest percentage of cell adhesion. The
microparticles with the highest encapsulation of extract were homogenized in the matrix solution
and oven dried to form a film. The matrix formulas were characterized and evaluated including cell
viability, in vitro release, morphology analysis, degradation rate, and water absorption capacity.
The optimum cell viability and collagen content were found at concentration of 20 ?g/mL Aloe vera
and 750 ?g/mL extract, respectively. COS-lecithin (1:20) (w/w) microparticles provided the highest
encapsulation of 65.88 ± 0.09 %. The matrix base containing gelatin-PVA-chitosan (3:2:2.5) (w/w/w)
gave cell viability of 66.20 ± 9.95 %, in vitro release of 54.67 ± 9.37 % for 12 days, degradation rate
of 83.43 ± 8.63 % for 12 days, and water absorption capacity 124.79 ± 24.53 %. To conclude, the
matrix is a potential candidate to use for quantitative assay of collagen inducing substances in in
vitro cultured of fibroblast.
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