KAJIAN RESPON DENV-2 DAN DENV-4 TERHADAP NANOEMULSI KURKUMIN SECARA IN VITRO

• Main Author: Khansa Suada, Nadia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44280
• Institution: Institut Teknologi Bandung

Curcumin is an active compound of the Curcuma domestica that shows potential benefit in therapies including antivirus, and one of them is as antivirus dengue. Curcumin is able to inhibit dengue virus replication through cellular mechanisms such as through ubiquitin-proteasome system (UPS) and modulated enveloped virus. Curcumin has a low solubility in water, easily degraded by light and pH and is easily metabolized into an inactive form, causing low bioavaibility in the body. This is a major problem in the development of curcumin preparations. The dosage form used in this study was nanoemulsion, in which the preparation increased solubility, increased stability, and increased efficacy of curcumin. The aim of this study was to examine the physicochemical characteristics and the stability of curcumin into nanoemulsion using formulas in the previous study and evaluating the antiviral potential of curcumin in nanoemulsion compared with curcumin in DMSO in vitro by infected A549 (human lung ephithelial cell line) with virus DENV¬2 and DENV-4. The physical characteristics of the curcumin nanoemulsion were performed by determining the particle size, the polydispersion index and the potential zeta using the Particle Size Analyzer. Physical stability test is done by visual observation and chemical stability by determining the content using High Pressure Liquid Chromatography at temperature 25°C and 40°C. The cytotoxic effect of curcumin on cell A549 is determined by the value of CC50 using MTT method (3- [4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide). The antiviral effect of curcumin is determined by IC50 value using plaque assay. The results of characterization of globul size, polydispersion index and zeta potential were 43,6± 13,27 nm, 0,39±0,175 and -0,12±0,5 mV; morphology exhibits encapsulated curcumin in a spherical nanoemulsion system of 100 nm in size. Storage of curcumin nanoemulsion without the addition of preservatives at temperature 25°C and 40°C showed a tendency to be contaminated by microbes. The CC50 of curcumin nanoemulsion and curcumin solution: 52,278 ?g/ml and 61,536 ?g/ml, respectively. Curcumin can decrease viral titers DENV-2 and DENV-4 with IC50 curcumin in nanoemulsion and the respective solutions are 2,606 ?g/ml, 4,03 ?g/ml, 15,134 ?g/ml, and 17.236 ?g/ml. The results showed that DENV-2 and DENV-4 viruses responded positively to curcumin, both in the form of nanoemulsion and solutions. Potential curcumin as antiviral DENV-2 and DENV-4 both in the form of nanoemulsion and the solution is comparable, so that nanoemulsion is potentially used as an alternative to curcumin delivery as a dengue antiviral.