KAJIAN RESPON DENV-1 DAN DENV-3 TERHADAP NANOEMULSI KURKUMIN SECARA IN VITRO

• Main Author: Nabila, Najwa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44281
• Institution: Institut Teknologi Bandung

Dengue fever is a disease caused by Dengue virus belonging to Flaviviridae family with the genus Flavivirus. There is no antiviral compound that can be utilized in the treatment of dengue fever. Curcumin is an isolate of Indonesia's natural ingredients which is known to have a good therapeutic effect for healing of various diseases, and having antiviral activity. Although the potential of curcumin for various diseases is promising, however clinically curcumin can’t provide good efficacy. This is caused by poor physicochemical characteristics, such as low solubility, and easily degraded by various factors such as light and air. The objectives of this research were to study physicochemical characteristics and curcumin stability through encapsulation in nanoemulsion using previous established formula, and also evaluated the antiviral potency of curcumin in nanoemulsion in vitro using A549 cells infected with DENV-1 and DENV-3. The physical characterization of curcumin nanoemulsion was performed by determination of particle size, polydispersity index, zeta potential, using Particle Size and Zeta Potential Analyzer; morphological characterization of curcumin nanoemulsion was done using Cryo-Transmission Electron Microscope; physical stability was determined by visual observation, and the curcumin content was determined using High Performance Liquid Chromatography. Evaluation of antiviral potency was done by determination of CC50 value by MTT method [3- (4.5-Dimethylthiazole-2-yl) -2.5- Difenyltetrazolium Bromide]; IC50 value by Plaque Assay method at full-treatment condition; statistical relationship between response to curcumin solution and curcumin nanoemulsion in vitro. Determination of CC50 value was done using microplate reader with wavelength 570 nm, IC50 value was determined by setting the amount of virus virus manually, and statistical analysis of two different preparation response was done by paired-t-test method. Curcumin nanoemulsion has size of 40.85±0.919 nm, Polidispersity Index of 0.366±0.165 and charge of -7.039±0.532 mV; spheric and uniform in size; and stable at 4oC after 3 months storage. The CC50 value of curcumin nanoemulsion was 52.967 ?g/ml, while IC50 was 0.969 ?g/ml for DENV-1 and 22.619 ?g/ml for DENV-3. It can be concluded that DENV-1 and DENV-3 were very responsive to curcumin. The antiviral potency of curcumin in the form of nanoemulsion and solution against virus DENV-1 and DENV-3 was proportional. Hence, curcumin nanoemulsion was promising as an alternative form for dengue virus infection treatment.