VALIDASI UJI POTENSI ANTIBIOTIK NISTATIN DENGAN METODE DIFUSI AGAR MENGGUNAKAN PENCADANG KERTAS

• Main Author: Rahmadani, Febi
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44320
• Institution: Institut Teknologi Bandung

A reduction in antimicrobial activity may not be adequately demonstrated by physicochemical methods. To overcome this problem, microbiological testing is conducted by determining the potency of antibiotic. One of the antibiotics required to be tested in microbiology is nystatin. According to pharmacopoeia, diffusion method is carried out by using a cylinder as a place of antibiotic sample. While in this research used paper disc therefore need to be validated. The aim of this research was to validate the use of paper disc in determination antibiotic potency of nystatin against Saccharomyces cerevisiae. The determination of antibiotic potency of nystatin was performed by diffusion method, using 5+1 test model , 5 dose of standards and 1 dose of test solution, and 3+3 test model, 3 dose of standards and 3 dose of test solution. The validation parameters specified were linearity, accuracy, precision, and quantization limit and detection limit. The results showed that the testing of nystatin potency by using paper disc displayed high linearity, accuracy, and precision. The model of 5+1 level gave linear result with coefficient correlation was 0,958 at concentration range of 80-195 U, recovery was 101,015% and precision inter-day and intra-day with coefficient variation 1,246 and 1,354. The model of 3+3 gave linear results with a coefficient correlation was 0.997 at concentration range of 40-160 U, recovery was 98.534% and precision inter-day and intra-day with coefficient variation 0.833 and 0.731. From this result results can be concluded that the use of paper disc in the agar diffusion method with the both test models gave appropriate result with the validation requirements, therefore it can be used for determining the antibiotic potency of nystatin.