AKTIVITAS ANTIMIKROBA DAUN MUDA DAN DAUN TUA TEMBAKAU (Nicotiana tabacum L.)

• Main Author: Christyani, Gabriella
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44347
• Institution: Institut Teknologi Bandung

Tobacco (Nicotiana tabacum L.) is one of commodity plant in Indonesia, but until now its utilization is limited for cigarettes production. Cigarette has negative effect on health. Empirically, tobacco is used for many other purposes, one of them as an antiseptic. This study aims to prove and compare the antimicrobial activity of unripe and ripe leaves of tobacco to confirm its use as antiseptic. Preparation of unripe and ripe leaves extract was carried out by extraction of crude drug by reflux using ethanol 96%. The extract was then fractionated by liquid-liquid extraction method using n-hexane and ethyl acetate. Antimicrobial activity of ethanol extracts, ethyl acetate fractions, and water fractions were tested with paper disc diffusion method at concentrations 5, 10, and 20% against Escherichia coli, Staphylococcus aureus, and Candida albicans. The result showed that the ethyl acetate fractions has inhibitory effect on Escherichia coli and Staphylococcus aureus but not on Candida albicans. There was no inhibitory effect observed in ethanol extracts. The water fraction of ripe leaves also have inhibitory effect againts Staphylococcus aureus. This study showed that unripe and ripe tobacco leaves had activity as an antimicrobial although not strong and there was no significant difference between antimicrobial activity of unripe and ripe leaves.