PENGARUH KOMBINASI PENGARUH KOMBINASI EKSTRAK ETANOL DAUN SIRIH (PIPER BETLE L.) DAN DAUN SENGGUGU (CLERODENDRON SERRATUM (L) MOON.) TERHADAP BAKTERI PENGINFEKSI LUKA OPERASI ORGAN DALAM
One of the causes of increasing mortality rate in Indonesia is infectious diseases. Organ surgical site infection (SSI) is nosocomial infection that caused by Pseudomonas aeruginosa and Enterococcus faecalis bacteria. Based on previous research, betel leaves (Piper betle), Clerodendron leaves (Clero...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/44348 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | One of the causes of increasing mortality rate in Indonesia is infectious diseases. Organ surgical site infection (SSI) is nosocomial infection that caused by Pseudomonas aeruginosa and Enterococcus faecalis bacteria. Based on previous research, betel leaves (Piper betle), Clerodendron leaves (Clerodendron serratum), Kaempheriae rhizomes (Kaempferia galanga), and onion (Allium cepa) showed antibacterial activity against bacteria that cause site surgical infections. This study revealed the antibacteria activity of ethanolic extracts of these plants and defined the combined effect of betle and clerodendron ethanolic extracts against Pseudomonas aeruginosa and Enterococcus faecalis bacteria. The crude drug of betel leaves, clerodendron leaves, kaempheria rhizome were extracted by reflux method using 96 % ethanol as a solvent. While fresh onion was extracted by maceration method using 96 % ethanol as a solvent. The four extracts were tested for antibacterial activity by microdilution assay on Pseudomonas aeruginosa and Enterococcus faecalis bacteria to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) with HCl tetracycline as a standard. Then the combined effect of extracts were tested with paper strip test, time kill curve, and checkerboard assay. Microdilution results showed the MIC of ethanolic extract of betel leaves against Enterococcus faecalis and Pseudomonas aeruginosa were 128 ?g/mL and 512 ?g/mL, respectively. The MIC of ethanol extract of clerodendron leaves against the same beacteria were 512 ?g/mL and 1024 ?g/mL. The ethanolic extract of kampheria rhizomes and onion had no antibacterial activity. The ethanolic extracts of betel and clerodendron were proceed to next step to determine their combined effect. The results showed additive effect with fractional inhibitory concentration (FIC) index was equal to 1.
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