PENGKAJIAN ANTIHIPERURISEMIA EMULSI MINYAK HATI IKAN KOD PADA MENCIT SWISS WEBSTERJANTAN

• Main Author: Emasurya Indrajaya, Stefiani
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44350
• Institution: Institut Teknologi Bandung

Cod liver oil emulsion is believed of being able to prevent uric acid accumulation. This research was held to examine antihyperuricemic activitywith in vivoand develop antihyperuricemic method with in vitroof cod liver oil emulsion. In vivo method was accomplished using Swiss Webster male mice inducted with high purine meal 100 mg/kg BW chicken liver suspense, together with drugs or cod liver oil emulsion through 20 days. Control group consumed high purine meal. Allopurinol group consumedhigh purin meal and 26 mg/kg BW of allopurinol. Vitamin A group consumedhigh purine meal and 650 IU/kg BW of vitamin A. Low dose, medium dose, and high dose group consumedhigh purine meal and 150 mg/kg BW, 300 mg/kg BW, and 450 mg/kg BW of cod liver oil emulsion. Strongest antihyperuricemic capability was shown by dose of 150 mg/kg BW with 57,04% degradation of uric acid compared to control group.In vivo showed that cod liver oil emulsion given together with high purine meal had antihyperuricemic effect. In vitro method was accomplished using pure uric acid and purine base with rat liver homogenate as xanthine oxidase enzyme source added. Invitro showed cod liver oil emulsion degraded uric acid in 0,525 mL medium mixture of 0,075 mg of uric acid and 0,011 mL cod liver oil emulsion; cod liver oil emulsion prevented uric acid establishment from purine in 0,725 mL medium mixture of 0,200 mg of purin, 0,066 gram of liver homogenate, and 0,016 mL of cod liver oil emulsion.