PENGUJIAN TOKSISITAS IN VITRO EKSTRAK DAN FRAKSI DARI DAUN JAMBU AIR (SYZYGIUM AQUEUM) DAN KULIT BUAH DELIMA (PUNICA GRANATUM) TERHADAP SEL VERO

• Main Author: Mutia, Cindra
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44355
• Institution: Institut Teknologi Bandung

Syzygium aqueum and Punica granatum are plants commonly found in Indonesia. Flavonoids from Syzygium aqueum leaves and Punica granatum peels are known to have antioxidant effect for human body. Flavonoid content as a source of antioxidants makes these plants widely used for preventation and treatment several diseases. Traditionally, Syzygium aqueum leaves and Punica granatum peels are commonly used as antibiotic, antidiabetic, and diarrheae. This study aims to determine in vitro toxicity effect of Syzygium aqueum and Punica granatum extract and fraction using Vero cell lines. Crude drug of Syzygium aqueum was extracted by maceration, while crude drug of Punica granatum was extracted by reflux. Both crude drugs were extracted using continuous extraction method and use solvents with increasing polarity which were n-hexane, ethyl acetate, and ethanol. Extracts were monitored by thin layer chromatography (TLC). The ethanol extract from Syzygium aqueum and Punica granatum was fractionated by liquid-liquid extraction using n-hexane, and ethyl acetate as solvents. Fractions were monitored by thin layer chromatography (TLC). Toxicity effect of Syzygium aqueum and Punica granatum extracts and fractions on Vero cells were measured using Alamar blue. Extracts and fractions of Syzygium aqueum leaves and Punica granatum had no toxicity effect against Vero cell at the lowest concentration 2 µg/ml and at the highest concentration 1024 µg/ml. Based on research, n-hexane, ethyl acetae, and ethanol extracts had no toxicity effect against Vero cell lines. N-hexane, ethyl acetate, and water fractions had no toxicity effect against Vero cell lines.