ISOLASI SENYAWA FLAVONOID DARI DAUN GARUT (MARANTA ARUNDINACEA L.)

• Main Author: Maulina Putri, Eva
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44362
• Institution: Institut Teknologi Bandung

Arrowroot (Maranta arundinacea L.) is a plant native to America and distributed in tropical countries, including Indonesia. The plant has often been be used in traditional medicines. Studies showed pharmacological effect of arrowroot leaves as antidiarrhea. Other studies have also shown the presence of rutin and quercetin in the leaves. However, there have been no studies concerning isolation of flavonoid compounds from the leaves. In this study, a flavonoid compound other than rutin and quercetin was isolated. Crude drug was extracted by reflux using ethanol as solvent, filtered and concentrated. The extract was monitored by thin layer chromatography (TLC). Chlorophyll separation was done by adding hot water into the extract and filtered. The filtrate was fractionated by liquid-liquid extraction method, using n-hexane and ethyl acetate solvents, yielded n-hexane, ethyl acetate, and water fractions. All fractions were monitored by TLC. The water fraction was subfractionated by classic column chromatography using cellulose as stationary phase with an isocratic mobile phase. The subfractions were monitored by TLC. A selected subfraction was then purified by preparative TLC, giving one compound to be tested for its purity by TLC using three different mobile phases and two-dimensional TLC. The isolate was characterized by ultraviolet-visible spectrophotometry, ammonia vapour, and two-dimensional paper chromatography. The isolate was predicted to be an isoflavon 7-O-diglycosides lacking a free 5-hydroxyl group with maximum absorbances at 274 nm and 321 nm.