ISOLASI FLAVONOID DARI DAUN PANDAN WANGI (PANDANUS AMARYLLIFOLIUS ROXB.)

• Main Author: Yuliana, Rina
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44371
• Institution: Institut Teknologi Bandung

The plant of “pandan wangi” is commonly found in Southeast Asia and its leaves have been used for therapeutic purposes. The flavonoid content in the leaves has been researched in vivo as antidiabetic. In this research, a flavonoid compound was isolated and characterized. The initial steps of this research were preparation and characterization of crude drug, followed by phytochemical screening. The leaves were extracted by reflux using ethanol as solvent. The ethanol extract was concentrated using rotary evaporator and yielded 185.53 g. Chlorophyll was removed by the use of hot water and the extract was fractionated by liquid-liquid extraction using modified Charaux-Paris method with n-hexane and ethyl acetate as solvents. This process yielded n-hexane fraction of 0.39 g, ethyl acetate fraction of 5.02 g and water fraction of 75.39 g. The ethyl acetate fraction was subfractionated by radial chromatographic system with gradient eluent from n-hexane – ethyl acetate (1:1) until methanol and yielded 85.40 mg of subfraction A. One compound in subfraction A was purified by preparative thin-layer chromatography (TLC) and yielded 33.90 mg of compound A2. Purity test was done by two-dimensional TLC and ultraviolet-visible spectrophotometry. Characterization of the pure isolate by ultraviolet-visible spectrophotometry, shift reagents, two-dimensional paper chromatography, and fluorescence colour with ammonia vapor indicated the isolate A2 was a flavone with free hydroxyl groups at positions of carbon 5, 7, and 4’ and the hydroxyl at carbon 3 was substituted.