AKTIVITAS ANTIBAKTERI KOMBINASI MINYAK ATSIRI BATANG SERAI (CYMBOPOGON CITRATUS DC. STAPF) DENGAN EKSTRAK ETANOL KULIT BUAH DELIMA MERAH (PUNICA GRANATUM L.)

• Main Author: Stefani, Mellisa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44397
• Institution: Institut Teknologi Bandung

Bacterial infection is a disease that has a considerable number of events in Indonesia. Bacterial infections can be treated using antibiotics but the problems associated with antibiotics make infectious diseases still difficult to be treated. Development of new antimicrobial agents needs to be done and one can be derived from natural materials. In previous researches, the essential oils of Cymbopogon citratus and ethanolic extract of Punica granatum peel have good antimicrobial activity against some bacterias. The aim of this research was to determine antimicrobial effect of Cymbopogon citratus’s essential oil combined with ethanolic extract of Punica granatum peel. The method began with determination of Minimum Inhibitory Concentration (MIC) using microdilution method and determination of Minimum Bactericidal Concentration (MBC) from each antimicrobial substance. The combination effect was determined using paper strip and time kill curve. The MIC value of Cymbopogon citratus’s essential oil against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were 1024 ?g/mL, 1024 ?g/mL, >4096 ?g/mL, respectively. The M IC value of ethanolic extract of Punica granatum peel against E. coli, S. aureus, and P. aeruginosa were 512 ?g/mL, 512 ?g/mL, and 1024 ?g/mL, respectively. The MBC value of Cymbopogon citratus’s essential oil against E. coli, S. aureus, and P. aeruginosa were 4096 ?g/mL, 2048 ?g/mL, and >4096 ?g/mL, respectively. The MBC value of ethanolic extract of Punica granatum peel against E. coli, S. aureus, and P. aeruginosa were >2048 ?g/mL, >2048 ?g/mL, and >2048 ?g/mL, respectively. In the determination of MIC, bacterias that could be inhibited by both of these test solutions were E. coli and S. aureus so that the combination activity test was only performed on both bacteria. The combination of these two extracts gave an additive effect to E. coli and S. aureus with both extract had bacteriostatic action.