FORMULATION AND ANTIMALARIAL IN VITRO STUDY OF OLIGODEOXYNUCLEOTIDE NANOCARIER TARGETED OF eba-175 AND dhs

• Main Author: Permata Wijaya, Dina
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44439
• Institution: Institut Teknologi Bandung

The main problems in therapy malaria using conventional drugs are multidrug resistance and no specific target in parasite cell. Hence, treatment using nucleic acid specific target is needed. Antisense oligodeoxynucleotide (as-ODN) is a single strain nucleotide can attach mRNA that inhibit gene expression in specific sequence of Plasmodium falciparum. The aim of this research was to develop therapy using as-ODN targeted eba-175 and dhs. Using delivery system of nanoparticles to prevent enzymatic degradation and to facilitate the internalization into red blood cells. As-ODN was encapsulated into chitosan-PLGA and poloxamer nanoparticles was prepared using nanoprecipitation method. The antimalarial study of chitosan-PLGA with 5 and 10% poloxamer nanoparticles was determined in vitro test. The nanoparticles formed were characterized for particle size and polidispersity index. The results showed that particles size as- ODN targeted gene eba-175 nanoparticles were between 127,2 ± 0,1-145,2 ± 4,2 nm while dhs gene were between 111,4 ± 2,6-134,4 ± 4,5nm and polydispersity index was 0-0,35. The highest inhibition growth of schizont was obtained 0,5 µM as-ODN nanoparticles targeted dhs of 65,4%. While the highest decrease total concentration of RNA in Plasmodium falciparum was obtained as-ODN nanoparticles targeted dhs of 32,1%. Additionally, the inhibition growth of schizont as-ODN nanoparticles exhibited significantly different (p<0,01) than free as-ODN.