DESIGN OF CHITOSAN MICROPARTICLES-BASED MATRIX AS A DELIVERY SYSTEM OF SODIUM ASCORBYL PHOSPHATE FOR INCREASING THE GROWTH OF FIBROBLAST CELLS

• Main Author: Syafitri, Erga
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44442
• Institution: Institut Teknologi Bandung

Background and objective: The completion of skin wound healing process is primarily determined by the activity of fibroblast cells. This process requires a wound closure matrix that can facilitates cell adhesion, proliferation and collagen formation. The goal of this study is to develop a biodegradable microparticlebased chitosan matrix formula containing sodium ascrobyl phosphate (SAP) for in vitro collagen production testing. SAP is a derivative of ascorbic acid, which can increase the production of collagen in fibroblast cell. Aloe vera was added to the matrix for inducing the cell adhesion and proliferation of cells in the early step. Methods: SAP and aloe concentrations were optimized using BALB/c 3T3 grown in treated well. Cell viability testing used the resazurin method utilizing the reduction of resazurin to resorufin by enzymes of living cells. The collagen content teting using the picro sirius red method that will bind to collagen forming a colored complex. Subsequently, SAP was encapsulated within microparticle prepared by double emulsion-solvent evaporation and solidified using crosslinking agent of sodium tripolyphosphate (STPP) or lecithin. The microparticle was incorporated into the matrix consisted of a mixture of various polymers such as gelatin, PVA, and chitosan. The matrix was selected to have the highest percentage of cell adhesion and 2 weeks of-degradation period. The matrix formulas were characterized and evaluated, including: cell viability, in vitro release, water absorption capacity, degradation rate, SAP stability against the matrix preparation process, and morphological analysis. Result: The optimum concentration of SAP that increased collagen content was 100-500?g/mL and the optimum concentration of aloe that increased cell viability was 20?g/mL. The best microparticle formula contained chitosan-lecithin 1: 40 (w/w) with encapsulation efficiency of 62.65 ± 0.51%. The matrix base contained gelatin-PVA-chitosan at the ratio of 3: 2: 2.5 (w/w/w) and SAP gave the cell viability of 79.09 ± 1.94% and controlled the release of SAP of 63.72 ± 0.04% for 12 days. The microparticle-based matrix had the water absorption capacity of 132.52 ± 6.86%, degraded of 92.98 ± 8.00% and SAP released of 20.96 ± 0.03% for 12 days. The microparticle-based chitosan matrix increased the stability of SAP during preparation conditions of matrix that involved heating and UV irradiation. Conclusion: This research has successfully developed matrix base for wound dressing containing SAP and microparticles that can be a potential candidate to vii use for quantitative assay of collagen inducing substance duringin vitro culture of fibroblast.