PROTEIN REKOMBINAN SUB-UNIT L TIROSINASE DARI JAMUR AGARICUS BISPORUS SEBAGAI PEMBAWA UNTUK SISTEM PENGHANTARAN ORAL: KAJIAN AWAL PEMBERIAN BERULANG PADA MENCIT GALUR SWISS WEBSTER

• Main Author: Efthyani, Alida
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44543
• Institution: Institut Teknologi Bandung

Oral drug delivery system is widely developed, but low bioavailability of the drug is the major problem. Thus, research nowadays focuses on the development of excipient or drug carrier to improve bioavailability by enhancing gut penetration. Light Sub-Unit Mushroom Tyrosinase (LSMT) is a protein that was first discovered in button mushrooms (Agaricus bisporus). Tyrosinase has light sub-units and heavy sub-units. These sub-units show different activity, H sub-unit shows tyrosinase enzyme activity, while L sub-unit shows no activity as tyrosinase enzyme. The L sub-unit (LSMT) exhibits a lectin-like structure, a structure that highly similar with HA 33 from Clostridium botulinum and CNL from Clitocybe nebularis which have been used as drug carriers to improve bioavailability. The lectin-like structure is able to interact with glycosylated structures on the epithelial surface of small intestine that conducts to endocytosis or transcytosis. However, protein application may have negative impact due to the immunogenicity and toxicity. As an early stage to ensure safety use, animals response to LSMT was evaluated after intraperitoneal administration once a week to healthy male and female Swiss Webster mice to simply avoid the complexity of oral absorbtion. The experiment was performed for 12 weeks. Several parameters were observed including body weight, serum IgG, organ indices, and organ histology. Dot Blot method was used to evaluate the antibody IgG generation as an immune response against LSMT. After 12 weeks of administration, animals were sacrificed and primary organs (liver, spleen, heart, lungs, and kidney) were isolated to assess organ indices number and microscopic observation. The result of Dot Blot method indicates that there was no specific antibody generated as a response after 12 weeks of LSMT administration. Based on Independent-Samples T Test statistical analysis of body weight and organ indices, significant difference was considered if p<0,05, the difference between test and control groups were not significant statistically. Microscopical observation showed no significant difference between test and control groups primary organs.