FORMULASI NANOKARIER KITOSAN-POLOKSAMER MENGANDUNG as-ODN BERTARGET GEN dhs DAN UJI AKTIVITAS ANTIMALARIA FALCIPARUM SECARA IN VITRO

• Main Author: Haqqun Agniyati, Ingguma
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44557
• Institution: Institut Teknologi Bandung

ABSTRACT Malaria is one of an epidemic disease at tropical zone caused by Plasmodium parasite. Plasmodium delivered by female Anopheles sp. Most malaria disease was caused by Plasmodium falciparum 3D7. Because of high mortality rate caused by malaria disease and drugs resistance of anti-malaria, we should develop drug correctly to solve those problems. Antisense - oligodeoxynucleotide (as-ODN) is single-strain DNA that was designed for specific targeted mRNA sequence that causes trouble, damage, or disablement. Therapy of as-ODN is limited because of the fast clearance from blood circulation and the degradation from serum nuclease. Thus, we need nanocarrier that are able to deliver the as-ODN into target. The aim of the study is to formulate and do in vitro antimalarial activity assay of cell culture infected by Plasmodium falciparum. The formula was prepared by ionic gelation using tripolyphosphate as crosslinking agent (TPP), contain chitosan and poloxamer with the particle size of target is less than 200 nm. Nanocarrier characterization consists of particle size, polidispersity index, and zeta potential. PCR product size confirmation was determined to choose correct primer that generate the same size as theoretical size. Activity test was determined by calculating inhibition percentage after 48 hours and analyzed microscopically. Inhibition percentage was determined by calculating the number of schizonts against 200 asexual parasites. Optimum formula obtained was 1 mg/mL chitosan, 50 mg/mL poloxamer, and 0.75 mg/mL TPP. Characterization of nanocarriers obtained as-ODN 0.5 ?M were mean particle size of 157.7 ± 8.5 nm, polydispersity index of 0.311 ± 0.021, zeta potential of 0.145 ± 0.106 mV. Primer that had correct product size was dhs f4r4 primer. as- ODN loaded nanocarrier had percentage of inhibition of 39.9% from normal control (p<0.05) and more effective than as-ODN 0.5 ?M.