FORMULASI, KARAKTERISASI, DAN UJI AKTIVITAS ANTIMALARIA NANOKARIER YANG MENGANDUNG OLIGODEOKSINUKLEOTIDA DENGAN TARGET GEN EBA175 TERHADAP PLASMODIUM FALCIPARUM 3D7

• Main Author: Kunto Putri, Irene
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44562
• Institution: Institut Teknologi Bandung

In therapy of malarial, the main problems in treatment using conventional antimalarial drugs are resistance and non-spesific target. As an alternative strategy, a new malaria therapy using nucleic acid specific to the target gene can be solution to overcome the problem. ODN (oligodeoxynucleotide) is able to inhibit gene expression in specific sequences in the parasite Plasmodium falciparum, such as EBA175 gene. The use of nanoparticles system to deliver ODN is essential to mediate transport across membrane parasite of cells. ODN nanoparticles prepared with chitosan and STPP with ionotropic gelation method. Antimalarial activity studies release profile was determined with in vitro test, then parasite cell was observed under microscope at 1000 times magnification. Physics character of the particle size at dosage levels of ODN nanoparticles concentration 0.05 µM is 141.20 ± 35,20 nm with a polydispersity index of 0.25 ± 0.07, while for the 0.5 µM level is 154.53 ± 22.03 nm with a polydispersity index of 0.29 ± 0.03. Measuring potential zeta of ODN nanoparticle concentration 0.5 µM is 0.16 ± 0.23 mV. The percentage of malarial parasites cell growth inhibition by free ODN concentration 0.5 µM is 21.2%. While, the inhibition percentage by ODN nanoparticles concentration 0.05 µM is 58.6%. The amount is better if compared with free ODN concentration 0.05 µM is 15.7%. Inhibition of gene expression can be determined with conventional PCR. There were two primer pairs for gene EBA175 and one primer pair as housekeeping gene that is 18s rRNA. Only one primer pair that showed single amplicon and correct amplicon size that is F1R1 primer.