FORMULASI ADSORPSI ACEMANNAN DALAM NANOPARTIKEL KITOSAN UNTUK SISTEM PENGHANTARAN RIFAMPISIN PADA TUJUAN PENGGUNAAN ORAL

• Main Author: Soraya Choirunissa, Eldi
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44571
• Institution: Institut Teknologi Bandung

Tuberculosis is a respirational infection disease caused by Mycobacterium tuberculosis (Mtb). Mtb possesses mannose capped lipoarabinomannan (ManLAM) as ligan that can be recognized by mannose receptors on macrophage. The goal of this study is to develop rifampicin formulation in chitosan nanoparticle as active targeting rifampicin to macrophage. Acemannan was isolated using alcohol precipitation method. Chitosan nanoparticles were formed by ionotropic gelation and acemannan was adsorbed on the particle surface prior to crosslinking using sodium tripolyphosphate (STPP). To acquire the optimum size and distribution of particle, several parameters were optimized including the mixing method, pH conditions, stirring speed, concentrations of sodium tripolyphosphate, and acemannan concentration. The preparation was then evaluated by measuring the size and polydispersity index of nanoparticles, particle charge and entrapment efficiency of rifampicin. The rendemen of acemannan obtained from the alcohol precipitation method was 59%. The optimum nanoparticle formulation was obtained by dropping 6 mg/mL of STTP solution into chitosan solution at pH 4 with stirring speed 800 rpm. The optimum concentration of rifampicin was 7.5 mg/mL and acemannan was 10 mg/mL. Polysorbate 80 20% was used to emulsify ethyl acetate solution of rifampicin in chitosan-acemannan solution. Characteristic of rifampicin loaded chitosan-acemannan nanoparticles was in the range of particle size 546.10 ± 22.94 nm, polydispersity index 0.240 ± 0.02, zeta potential value +1.22 ± 0.06 mV and the entrapment efficiency was 28.80 ± 2.23 %.