FORMULASI KOMBINASI RIFAMPISIN DAN ARTINM DALAM SISTEM NANOPARTIKEL KITOSAN UNTUK TUJUAN PENGGUNAAN ORAL

• Main Author: Dewi Intani, Rizka
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44578
• Institution: Institut Teknologi Bandung

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis which can damage many organs, mainly affect the lungs. Rifampicin as the first line of antituberculosis drug has several problems related to its stability, solubility, and bioavailability towards the presence of other antituberculosis drug. Artin-M is a lectin from jackfruit (Artocarpus integrifolia) seeds which has immunostimulant activities by inducing immune cells with glycosylated receptor on the surface such as macrophages and dendritic cells to produce cytokines. This study aims to formulate rifampicin along with artin-M into nanoparticles for oral route of administration by using low molecular weight chitosan and glucomannan with target of particle size less than 600 nm. Nanoparticles were prepared by ionic gelation using sodium tripolyphosphate as crosslinking agent (STPP). The formula was optimized to provide the highest entrapment efficiency of artin-M and rifampicin with appropriate particle size. Artin-M was adsorbed to glucomannan solution in order to bind specifically, and subsequently incorporated into nanoparticles. Evaluation of nanoparticles consisted of particle size, polidispersity index, zeta potential, entrapment efficiency of rifampicin, hemagglutination test and SDS PAGE of Artin-M. The optimum formula was obtained from 20 mg/mL chitosan at pH 4 with the additions of 6 mg/mL STPP, 10 mg/mL glucomannan, 20% (w/v) polysorbate 80, 1.9 mg/mL artin-M to glucomannan ratio, 0.75% (w/v) of rifampicin, and at ratio of chitosan : STPP : glucomannan was 5 : 2 : 1. Nanopaticles were obtained in average particle size of 405.2 ± 7.02 nm, polydispersity index of 0.218 ± 0.006, zeta potential of +31.65 mV, rifampicin entrapment efficiency of 53.26 ± 0.34%, and hemagglutination test indicated that 100% of artin-M was entrapped within nanoparticles.