KONSTRUKSI pET-16b_rec DENGAN METODE MUTAGENESIS SITUS TERARAH SEBAGAI VEKTOR EKSPRESI UNTUK PROTEIN FUSI POLIHISTIDIN DI UJUNG KARBOKSI PADA SISTEM ESCHERICHIA COLI
One of the most frequently methods used to purify recombinant proteins is affinity column chromatography. Recombinant proteins can be efficiently purified by nickel affinity column when fused with polyhistidine residue on either N-terminal or C-terminal of proteins. Position of polyhistidine tags...
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Format: | Final Project |
Language: | Indonesia |
Online Access: | https://digilib.itb.ac.id/gdl/view/44649 |
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Institution: | Institut Teknologi Bandung |
Language: | Indonesia |