PENGEMBANGAN METODE ANALISA SIMULTAN ALFA ARBUTIN DAN HIDROKUINON DENGAN KROMATOGRAFI CAIR KINERJA TINGGI PADA SAMPEL KRIM KOSMETIK

• Main Author: Marsha Claudia, Eleny
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44950
• Institution: Institut Teknologi Bandung

Hydroquinone is a skin whitening compounds that is banned in Indonesia based on BPOM regulation. Alpha arbutin is being developed as an alternative compounds to replace hydroquinone. Alpha arbutin, that has a basic structure of hydroquinone, are naturally found in plants. This compound is hydrolyzed to hydroquinone both in vivo and in vitro. The research was conducted to obtain an optimal method in the simultaneous determination of alpha arbutin and hydroquinone as a result of hydrolysis using HPLC. The system was being optimized with variation of mobile phase composition as well as the pH level of phospate buffer. The system was analyzed using a LiChrospher® RP-????18 10?????(4 ?250 ??) as column, methanol pro HPLC: H3PO4 - KH2PO4 (10:90) at pH 4 as mobile phase, flow rate of 1 mL/min, and UV-Vis detector at 281 nm wavelength detection. The retention for alpha arbutin and hydroquinone were 4.43 and 6.12 minutes. Linearity values obtained for linear curve of alpha arbutin was 0.998 with limits of detection and quantification were 0.47 and 1.57 ????ã/?????respectively. The value of linearity for linear curve of hydroquinone was 0.998 with limits of detection and quantification were 0.45 and 1.51 ????ã/?????respectively. The coefficient of variation for intraday precision test were 1.038; 1.063; 0.537 % for alpha arbutin and 0.280; 0.167; 1.465 % for hydroquinone. The coefficient of variation for interday precision test were 1.838; 0.400 % for alpha arbutin and 1.085%; 0.355% for hydroquinone. Percent recovery of alpha arbutin and hydroquinone in creams simulation was in range of 97.12-100.37 % and 97.14 -102.30 %. The concentration of alpha arbutin found in five different cream samples varied from 0,219-0,357 %b/b. It can be concluded that the HPLC method can be used for simultaneous analysis of alpha arbutin and hydroquinone.