VALIDASI UJI POTENSI ANTIBIOTIK KANAMISIN SULFAT METODE DIFUSI AGAR DENGAN MODEL PENGUJIAN 3+3 DAN 5+1 MENGGUNAKAN PENCADANG KERTAS

• Main Author: Dian W P, Kartika
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44952
• Institution: Institut Teknologi Bandung

Kanamycin is an aminoglycoside bactericidal antibiotic isolated from Streptomyces kanamyceticus. It has board spectrum activity, and active against gram positive, negative bacteria, and some mycobacterium. Determination of its potency in pharmaceutical formulation can be done with agar diffusion bioassay. Agar diffusion has some design of assessment, including 5+1 with 5 dose of standards and 1 dose of test solution, and 3+3 with 3 dose of standards and 3 dose of test solution. The use of paper disc for agar diffusion bioassay has not been previously reported. Thus, this paper reports validation of 5+1 and 3+3 agar diffusion bioassay using paper disc method to quantify kanamycin sulfate in pharmaceutical samples. The strain Staphylococcus aureus ATCC 6538 was used as the test microorganism, and the results of the proposed bioassay displayed high linearity, precision, and accuracy. All the potency with 5+1 model result were found to be linear (r >0.99) in the range of dose 6.4-15.6 µg, accurate (recovery 101.6%), and precise (coefficient of variance inter-day 2.2% and intra-day 4.4%). All the potency with 3+3 model also found to be linear (r >0.99) in the range of dose 3.2-12.8 µg, accurate (recovery 101.4%), and precise (coefficient of variance inter-day 4.5% and intra-day 2.6%). The results validated the proposed microbiological assay, which allows reliable quantitation of kanamycin sulfate in pharmaceutical samples.