PENGEMBANGAN METODE PENETAPAN KADAR FUROSEMIDA DAN SPIRONOLAKTON DALAM SEDIAAN TABLET DENGAN METODE SPEKTROFOTOMETRI ULTRAVIOLET DERIVATIF

• Main Author: Sugiharti, Dewi
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44953
• Institution: Institut Teknologi Bandung

Furosemide and spironolactone is a drug combination of active substances that act as a diuretic to treat hypertension. Furosemide and spironolactone have chromophore group that can absorb light at wavelengths that are close together. Derivative UV spectrophotometry can be used to assay mixture of active substance that has a wavelength that is contiguous with good precision and accuracy. The purpose of this research was to develop a methode of assay furosemide and spironolactone using spectrophotometry UV derivative. Furosemide was measured at second order derivative at a wavelength of 288 nm and spironolactone was measured at first order derivative at a wavelength of 251 nm. Validation of method includes linearity test, limit of detection, limit of quantitation, precision intraday, precision interday, and accuracy. Measurements of furosemide on the second order derivative, obtained regression coefficient of 0.999 with VXO amounted to 0.918% with a limit of detection of 0.202 µg/mL and limit of quantitation 0.605 µg/mL. Measurements of spironolactone on first derivative obtained regression coefficient of -0.9999 with VXO amounted to 0.355% with a detection limit of 0.087 µg/mL and limit of quantitation 0.267 µg/mL. Precision test intraday and interday showed coefficient variation as 1.17 and 0.91% for furosemide and 0.91 and 0.81% for spironolactone. Accuration test at a concentration 80, 100, and 120% showed recovery as 100.68; 99.83; and 99.70% for furosemide and 100; 99.73; and 100.12% for spironolactone. In conclusion, de rivative UV spectrophotometry method can be used to measure levels of furosemide and spironolactone with good precision and accuracy.