ISOLATION AND ANTIBACTERIAL ACTIVITY OF SOIL-DERIVED FUNGI FROM MALAYSIAN FOREST

• Main Author: Daniaal Radzali, Muhammad
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44959
• Institution: Institut Teknologi Bandung

Fungi are eukaryotic organisms that consist of unicellular organisms, namely molds and yeasts, and multicellular organism known as mushrooms. Fungi have major contribution towards medical field as they are widely used in developing antibiotics. The objective of this research is to isolate soil-derived fungi from Malaysian forest and test its antibacterial activity against Bacillus subtilis and Escherichia coli. The fungal were cultured and harvested in Sabouraud dextrose agar (SDA). Five fungal strains were isolated from the soil sample and each of them were examined macroscopically and microscopically to determine its morphological characteristic. They were then tested with its capability to produced secondary metabolites that may have antibacterial activity by fermentation process. The product of fermentation, the mycelium and liquid medium, were extracted by maceration and liquid-liquid extraction respectively using ethyl acetate. These extract was then used to determine the antibacterial activity against test bacteria. Based on the extract of LLE, SA 1 showed inhibition against Bacillus subtilis and Escherichia coli at 256 and 32 µg/mL respectively. On the other hand, SA 3 showed inhibition at 64 µg/mL against Bacillus subtilis. Based on the extract of maceration, SA 1 showed inhibition against Bacillus subtilis and Escherichia coli at 512 and 256 µg/mL respectively. SA 3 showed inhibition only against Bacillus subtilis at 256 µg/mL. However, SA 4 showed inhibition against Bacillus subtilis and Escherichia coli at 256 and 64 µg/mL respectively.