ANTIBACTERIAL ACTIVITY OF EXTRA VCO AND ORGANIC VCO AGAINST STAPHYLOCOCCUS AUREUS, STAPHYLOCOCCUS EPIDERMIDIS, AND ESCHERICHIA COLI

• Main Author: Arikrishnan, Nirranjenee
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44966
• Institution: Institut Teknologi Bandung

Virgin coconut oil is defined as the oil obtained from fresh coconut meat, without undergoing any chemical processes. Virgin coconut oil is mainly sold on the basis of its antimicrobial activity which is contributed to by its lauric acid. The aim of this thesis is to study and investigate the antimicrobial activity of two different types of commercial virgin coconut oil against human pathogenic bacteria such as Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis ATCC 12228, and Escherichia coli ATCC 8739. The antimicrobial activity of pure oil samples was tested and it is considered as 100% concentration. After 24 hours, the growths of the bacteria were observed by comparing the turbidity of all the samples with the controls and the minimum inhibitory concentration of antibiotic was determined using broth dilution method. The antibacterial activity of the oil sample were compared with Ampicillin standard, and their equivalency of antibacterial activity were determined. Growth of bacteria was only observed in sample containing E. coli as the content of the tube turned turbid and cloudy. As for S. aureus and S. epidermidis, inhibition of bacteria could be observed. Both the oil samples remained clear without the presence or growth of bacteria. Results were compared with positive control containing Ampicillin and bacteria and negative control containing only nutrient broth. The minimium inhibitory concentration (MIC) of Ampicillin in both S. aureus and S. epidermidis was 50 ppm, whereas for E. coli was 200ppm. As for the equivalency results, for S. aureus, 10 ?L of Organic VCO gives 12.9 mm inhibition diameter which is equivalent to 54.15 ppm of antiobiotic. Whereas for Extra VCO, 10 ?L of oil gives 12.0 mm inhibition diameter which is equivalent to 45.66 ppm of antibiotic. For S. epidermidis, 10 ?L of Organic VCO gives 13.7 mm inhibition diameter which is equivalent to 69.87 ppm of antiobiotic. Whereas for Extra VCO, 10 ?L of oil gives 14.2 mm inhibition diameter which is equivalent to 74.33 ppm of antibiotic. As the conclusion both Extra and Organic vco showed antibacterial activity against S. epidermidis and S. aureus. There was no inhibition found against E. coli. Ampicillin of 75 ppm was made as standard for both oil samples in S. epidermidis and Ampicillin of 50 ppm against S. aureus for both oil samples.