ISOLASI DAN UJI AKTIVITAS SENYAWA ANTIBAKTERI DARI KETEPENG KEBO (CASSIA ALATA L.)

• Main Author: Dewi Krisnamia, Elvirta
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/44992
• Institution: Institut Teknologi Bandung

Cassia alata L. or candle bush is a plant from the family of Caesalpiniaceae which is traditionally used for curing skin diseases. Acne, a skin disease, is estimated to affects 9,4% of the global population usually found on teens and young adults. Some researchers had found that candle bush has antimicrobial compounds however, the responsible compounds were still unknown. This research was aimed to determine the antibacterial compounds from candle bush. The crude drug of candle bush leaves was extracted by reflux using three solvents with increasing polarity, i.e. nhexane, ethyl acetate, and ethanol. All extracts were evaporated and monitored using thin layer chromatography (TLC). The extracts were tested for their antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, and Propionibacterium acnes using microdilution and bioautography methods. The ethanol extract was fractionated by liquid-liquid extraction, with n-hexane, ethyl acetate, and water. All fractions were evaporated and monitored with TLC. The ethyl acetate fraction was seperated with preparative TLC to give eight compounds. Two isolates showed antibacterial activity against Propionibacterium acnes. One of them showed a positive result to citroborate reagent, which was estimated as a flavonoid. The result of testing with NH3 vapor and ferric chloride reagents showed that the isolate was a flavonol with free 3-OH and without free 5-OH.