ISOLASI SENYAWA FLAVONOID UTAMA DARI DAUN BENALU (DENDROPHTHOE PENTANDRA (L.) MIQ.) YANG TUMBUH PADA POHON ANGSANA

• Main Author: Permata Sari, Intan
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45030
• Institution: Institut Teknologi Bandung

Mistletoe (Dendrophthoe pentandra L. Miq.) is one of parasitic plants which are included of family Loranthaceae. Mistletoe leaves are used as traditional medicine. Most of compounds which are contained in D. pentandra are flavonoids. Flavonoids have major activities like antiinflammation, antioxidant and antibacterial. Flavonoids in this mistletoe leaves hav been studied, but no studies that focus on mistletoe (Dendrophthoe pentandra L. Miq.) which grow on Angsana trees. This research is aim to isolate and characterize of mayor flavonoids from mistletoe leaves which grow on Angsana trees. The fresh mistletoe leaves are collected, sorted, cleaned, dried in an oven, and ground to produce crude drugs powder. Crude drugs were extracted by reflux method with methanol 50% in two hours. Extraction was performed three times and extract was monitored by Thin Layer Chromatography (TLC). Extract was concentrated by vacuum rotary evaporator until produced thick extract. Thick extract was dissolved in hot water and filtered, then filtrate was fractionated by Liquid-Liquid Extraction using petroleum ether, chloroform, and ethyl acetate respectively. These four fractions were monitored by TLC method. Based on qualitative analysis, most flavonoid compounds are contained in ethyl acetate fraction, because after sprayed by citroboric acid, appeared two spots with Rf 0,52 and 0,6. While in petroleum ether fraction an chloroform fraction there is no spot that appear, and in water fraction there is only a spot with Rf 0,52. Ethyl acetate fraction was separated by preparative TLC method. Purity of result of separation was tested by single development TLC method and preparative TLC. Purity test showed that result of separation is pure. The result of separation was characterized by UV-visible spectrophotometry with shift reagents. From the results of characterization, there was a main flavonoid compound which gave maximum absorbance in wavelength 256 nm (band II) and 349 nm (band I). The characterization with shift reagents and UV-visible spectrophotometry showed that this mayor flavonoid was predicted as flavon with hydroxy group on C5, C7, orto dihydroxy on ?ð?ã ????(????4’¬?¸ ????3’)????