PENGARUH HBx MUTAN T118N DAN K130M TERHADAP PEMBENTUKAN KOLONI SEL HepG2 SEBAGAI DASAR USULAN KONSELING

Hepatitis!B!Virus!(HBV)!belongs!to!Hepadnaviridae!family!which!infects!human!liver!cells.!HBV!can! cause! both! acute! and! chronic! infection.! The! chronic! infection! of! HBV! potentially! leads! to! complications!such!as!liver!cirrhosis!to!hepatocellular!carcinoma!(HCC).!The!development!of!HBV...

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Main Author:	Citra Permanasari, Silmi
Format:	Final Project
Language:	Indonesia
Online Access:	https://digilib.itb.ac.id/gdl/view/45210
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Institution:	Institut Teknologi Bandung
Language:	Indonesia

id	id-itb.:45210
spelling	id-itb.:452102019-11-28T10:52:18ZPENGARUH HBx MUTAN T118N DAN K130M TERHADAP PEMBENTUKAN KOLONI SEL HepG2 SEBAGAI DASAR USULAN KONSELING Citra Permanasari, Silmi Indonesia Final Project Hepatitis,B,Virus,,hepatitis,B,X,protein,(HBx),,HBx,T118N,,HBx,K130M,,hepatocelullar, carcinoma,(HCC),,colony,formation,assay.! INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/45210 Hepatitis!B!Virus!(HBV)!belongs!to!Hepadnaviridae!family!which!infects!human!liver!cells.!HBV!can! cause! both! acute! and! chronic! infection.! The! chronic! infection! of! HBV! potentially! leads! to! complications!such!as!liver!cirrhosis!to!hepatocellular!carcinoma!(HCC).!The!development!of!HBV! chronic!infection!is!predicted!to!be!associated!with!HBV!X!protein!HBx)!mutant!which!affects!host! cell!proliferation.!In!Indonesia,!HBx!T118N!and!K130M/V131I!are!the!most!frequently!occurring! HBx!mutant!in!patients!with!chronic!hepatitis!B,!cirrhosis!and!HCC.!HBx!K130M/V131I!has!been! studied!extensively!for!its!association!with!HCC.!However,!the!effect!of!HBx!T118N!and!K130M!in! liver!carcinoma!has!never!been!studied!before.!The!aim!of!this!study!was!to!determine!the!effect! of!HBx!T118N!and!K130M!expression!in!HepG2!cells!using!colony!formation!assay!and!the!results! were! used! as! suggestion! for! counseling.! Plasmids! pD609_HBx_wt,! pD609_HBx_T118N! and! pD609_HBx_K130M! were! first! isolated,! confirmed! and! then! transfected! into! HepG2! cells.! Succesful! transfection! was! detected! by! measuring! HBx! mRNA! expression! using! Reverse, Transcriptase0Polymerase, Chain, Reaction! (RTZPCR).! Selection! of! HepG2! cells! using! various! puromycin!showed!optimum!concentration!at!0,2!?g/ml.!Colony!formation!assay!was!performed! by!growing!transfected!cells!in!media!with!0,2!?g/ml!puromycin!for!14!days.!The!result!showed! that!number!of!colonies!formed!upon!HBx!T118N!and!K130M!expression!were!less!than!wildZtype! HBx! expression.! This! result! indicates! association! of! HBx! T118N! and! K130M! with! HCC.! This! information! was! further! used! as! counseling! suggestion! for! patients! and! health! professionals! to! increase!awareness!HBx!mutant!T118N!related!to!development!of!chronic!HBV!infection!into!liver! carcinoma.! % text
institution	Institut Teknologi Bandung
building	Institut Teknologi Bandung Library
continent	Asia
country	Indonesia Indonesia
content_provider	Institut Teknologi Bandung
collection	Digital ITB
language	Indonesia
description	Hepatitis!B!Virus!(HBV)!belongs!to!Hepadnaviridae!family!which!infects!human!liver!cells.!HBV!can! cause! both! acute! and! chronic! infection.! The! chronic! infection! of! HBV! potentially! leads! to! complications!such!as!liver!cirrhosis!to!hepatocellular!carcinoma!(HCC).!The!development!of!HBV! chronic!infection!is!predicted!to!be!associated!with!HBV!X!protein!HBx)!mutant!which!affects!host! cell!proliferation.!In!Indonesia,!HBx!T118N!and!K130M/V131I!are!the!most!frequently!occurring! HBx!mutant!in!patients!with!chronic!hepatitis!B,!cirrhosis!and!HCC.!HBx!K130M/V131I!has!been! studied!extensively!for!its!association!with!HCC.!However,!the!effect!of!HBx!T118N!and!K130M!in! liver!carcinoma!has!never!been!studied!before.!The!aim!of!this!study!was!to!determine!the!effect! of!HBx!T118N!and!K130M!expression!in!HepG2!cells!using!colony!formation!assay!and!the!results! were! used! as! suggestion! for! counseling.! Plasmids! pD609_HBx_wt,! pD609_HBx_T118N! and! pD609_HBx_K130M! were! first! isolated,! confirmed! and! then! transfected! into! HepG2! cells.! Succesful! transfection! was! detected! by! measuring! HBx! mRNA! expression! using! Reverse, Transcriptase0Polymerase, Chain, Reaction! (RTZPCR).! Selection! of! HepG2! cells! using! various! puromycin!showed!optimum!concentration!at!0,2!?g/ml.!Colony!formation!assay!was!performed! by!growing!transfected!cells!in!media!with!0,2!?g/ml!puromycin!for!14!days.!The!result!showed! that!number!of!colonies!formed!upon!HBx!T118N!and!K130M!expression!were!less!than!wildZtype! HBx! expression.! This! result! indicates! association! of! HBx! T118N! and! K130M! with! HCC.! This! information! was! further! used! as! counseling! suggestion! for! patients! and! health! professionals! to! increase!awareness!HBx!mutant!T118N!related!to!development!of!chronic!HBV!infection!into!liver! carcinoma.! %
format	Final Project
author	Citra Permanasari, Silmi
spellingShingle	Citra Permanasari, Silmi PENGARUH HBx MUTAN T118N DAN K130M TERHADAP PEMBENTUKAN KOLONI SEL HepG2 SEBAGAI DASAR USULAN KONSELING
author_facet	Citra Permanasari, Silmi
author_sort	Citra Permanasari, Silmi
title	PENGARUH HBx MUTAN T118N DAN K130M TERHADAP PEMBENTUKAN KOLONI SEL HepG2 SEBAGAI DASAR USULAN KONSELING
title_short	PENGARUH HBx MUTAN T118N DAN K130M TERHADAP PEMBENTUKAN KOLONI SEL HepG2 SEBAGAI DASAR USULAN KONSELING
title_full	PENGARUH HBx MUTAN T118N DAN K130M TERHADAP PEMBENTUKAN KOLONI SEL HepG2 SEBAGAI DASAR USULAN KONSELING
title_fullStr	PENGARUH HBx MUTAN T118N DAN K130M TERHADAP PEMBENTUKAN KOLONI SEL HepG2 SEBAGAI DASAR USULAN KONSELING
title_full_unstemmed	PENGARUH HBx MUTAN T118N DAN K130M TERHADAP PEMBENTUKAN KOLONI SEL HepG2 SEBAGAI DASAR USULAN KONSELING
title_sort	pengaruh hbx mutan t118n dan k130m terhadap pembentukan koloni sel hepg2 sebagai dasar usulan konseling
url	https://digilib.itb.ac.id/gdl/view/45210
_version_	1822270836089815040

PENGARUH HBx MUTAN T118N DAN K130M TERHADAP PEMBENTUKAN KOLONI SEL HepG2 SEBAGAI DASAR USULAN KONSELING

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