STUDY OF RENAL REPAIR MECHANISM OF CORN SILK (Zea mays L.), BINAHONG LEAVES (Anredera cordifolia (Ten.) Steenis), AND THEIR COMBINATION AGAINST RAT MODEL OF RENAL FAILURE

• Main Author: Ferdiana Adiwibowo, Levina
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45244
• Institution: Institut Teknologi Bandung

Background and purposes: Each time the body's cells produce metabolic waste products that must be removed from the body. The function is performed by the renal. If the renal function is decreased or even damaged, so-called renal failure, accumulation of toxic products and other balance disorders are happened. In those conditions, there is increased oxidative stress which is likely to exacerbate the initial conditions. Treatments of renal failure require high cost although the final results are not necessarily satisfactory. Past studies showed that both corn silk and binahong could improve renal function in rat model of renal failure. Therefore, this study was aimed to determine the effect of providing a combination of corn silk and binahong extract against renal damage and to study their effects on oxidative stress. Method: Rat model of renal failure was established through the provision of gentamicin 100 mg/kg bw intraperitoneally and piroxicam 3.6 mg/kg bw orally for 7 consecutive days. Test animals were divided into positive control group, corn silk extract test group of 75 mg/kg bw, binahong extract test group of 100 mg/kg bw, corn silk-binahong half dose extract test group, corn silk-binahong one dose extract test group, and negative control group. Extracts were given for 4 weeks. Parameters evaluation of renal function were serum creatinine, serum urea levels, renal index, and histology, whereas to measure levels of oxidative stress, the level of lipid peroxidation, catalase, and SOD (superoxide dismutase) activity were tested. Results: In the third week, it seemed that extract’s administration could decrease serum creatinine levels significantly. Decreased levels of serum urea were seen meaningful at the fifth week. Half dose combination extract test group give activity comparable to each single extract test group, but no augmentation effect was found significantly in the larger dose of combination extract test group. Each test group showed lower renal index value and better histology profile compared to the positive control group. Extract test groups shown to reduce the level of lipid peroxidation and increased catalase and SOD enzyme activity significantly. No significant differences were found between the test groups. Conclusion: Both corn silk and binahong extract could improve renal function in rat model of renal failure. Application of the half dose combination extracts showed effects comparable or slightly better than a single extract so it might have additive characteristic. Repair mechanism was caused by reduction of oxidative stress provided by each of the extracts and their combinations.