ANALYTICAL METHOD DEVELOPMENT TO DETERMINE THE COMPOSITION OF JAMU FOR HYPERURICEMIA TREATMENT USING TLC-DENSITOMETRY
Background and objective : Herbal drugs are increasingly used by community for the purpose of health care, prevention and disease treatment. Jamu for hyperuricemia treatment is one of the products that growing both its quantity and composition. Hence, it is necessary to do the quality assurance o...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/45316 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Background and objective : Herbal drugs are increasingly used by community
for the purpose of health care, prevention and disease treatment. Jamu for
hyperuricemia treatment is one of the products that growing both its quantity and
composition. Hence, it is necessary to do the quality assurance of the products.
Nowadays, the quality assurance is only conducted on the stage of its safety and
quality in general. The objectives of this study were to test the consistency of the
composition of the constituent materials in jamu for hyperuricemia treatment
using a valid method as alternative for quality control testing. Method:
The extraction was done using methanol with sonication. Each extract solution
was observed by Thin Layer Chromatography (TLC) to determine the spot that
used as marker. Furthermore, the validation was performed using TLCdensitometry. The valid method was used to evaluate the constituent compositions
of jamu for hyperuricemia treatment. Results : The validation parameters
evaluated were linearity, precision, recovery, robustness, Limit of Detection and
Limit of Quantification in bay-leaf extract as one constituent in jamu for
hyperuricemia treatment. The calibration graph was linear within the range of
50.02 –166.75 ?g/10 ?L with correlation coefficient of 0.9975. The Intra-day and
Inter-day precision were 0.62% and 2.32% respectively. The Limit of Detection,
Limit of Quantification and Recovery range respectively resulted as follows,
11.00 ?g/10 ?L, 36.68 ?g/10 ?L and 85.22-100.23%. Bay-leaves contained in the
samples were 22.19% (90.94% of the stated value). Conclusion: The developed
method was successfully applied as a quality control method to determine
bay-leaves extract contained in jamu for hyperuricemia treatment.
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