ISOLATION OF ANTIBACTERIAL COMPOUND FROM KALANGKALA SEED (Litsea angulata, Blume)

• Main Author: Priyanto Wibowo, Joko
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45356
• Institution: Institut Teknologi Bandung

Kalangkala seed (Litsea angulata Blume) was traditionally used for healing abscess in Kalimantan. This study aimed to determine antibacterial activity of kalangkala seed and to isolate antibacterial compound from kalangka seed. Extraction used soxhlet apparatus with n-hexane, ethyl acetate and ethanol solvent consecutively and also used supercritical fluid extraction (SFE) with CO2 liquid as solvent. N-hexane extract that gave stongest antibacterial activity was choosen to be fractionated by column chromatography then subfractionated by sentrifugal thin layer chromatography (TLC) and finally purified by preparative TLC to obtain compound 1. While SFE extract was purified by re-crystalitation to obtain compound 2. Antibacterial activity of each extract was tested using agar diffusion method to measure their zone of inhibition. The inhibition zone of n-hexane extract toward Staphylococcus aureus (11,28 mm + 0,21), Bacillus subtilis (12,31 mm + 0,22) and Escherichia coli (10,08 mm + 0,36), ethyl acetate extract toward B. subtilis (10,28 mm + 0,21) and E. coli (11,30 mm + 0,23), ethanol extract toward S. aureus (11,33 mm + 0,26), B. subtilis (11,25 mm + 0,24), E. coli (11,48 mm + 0,17) and methylcillin-resistent S. aureus (MRSA) (11,33 mm + 0,21), SFE extract toward B. subtilis (12,25 mm + 0,24) and E. coli (9,25 mm + 0,24). Minimum Inhibitory Concentration (MIC) of each compound was measured using microdillution method. MIC of compound 1 was 500 µg/mL toward S.aureus, 250 µg/mL toward B. subtilis and 1000 µg/mL to E. coli while MIC of compound 2 was 500 µg/mL toward S. aureus and B. subtilis and their structure were elucidated by spectroscopic data ( 1 H-NMR, 13 C-NMR, 2D-NMR), compound 1 was identified as campesterol and compound 2 was identified as tricaprin.