ISOLATION OF 5,7-DIHYDROXY, 6,8-DIMETHYL FLAVANON AND ANTIOXIDANT ACTIVITY WITH XANTHINE OXIDASE INHIBITORY ACTIVITY ASSAY OF WATER APPLE LEAVES EXTRACT (Syzygium aqueum Burm.f. Alston)
Xanthine oxidase produces free radicals when converting xanthine into uric acid. They cause oxidative stress, which cause several diseases. Usually, many compounds which inhibit xanthine oxidase also exist as an antioxidant. Some researches have proven several plants of Myrtaceae family that had...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/45357 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Xanthine oxidase produces free radicals when converting xanthine into uric acid.
They cause oxidative stress, which cause several diseases. Usually, many
compounds which inhibit xanthine oxidase also exist as an antioxidant. Some
researches have proven several plants of Myrtaceae family that had antioxidant
activity, but there are still very lack of informations that those plants have
xanthine oxidase inhibitory activity. This aim of the research was to determine the
antioxidant activity and xanthine oxidase inhibitor activity from several plants of
Myrtaceae family. Then, from the selected plants the active compound was
isolated. In order to achieve these objectives, the isolation steps of active
compound have been done trough a series of separation methods included
extraction, fractionation, detemination of antioxidant activity with radical
diphenyl picrylhydrayl/DPPH scavenging method, determination of xanthine
oxidase inhibitory activity, purification and characterization of active compound.
Extraction was performed by Soxhlet apparatus, followed by fractionation and
purification by vacum liquid chromatography and radial chromatography. The
active compound was characterized using spesific spraying reagents, Ultraviolet-
Visible spectrophotometry, Nuclear Magnetic Resonance (NMR)-
1
H, and NMR13
C. From this study, the methanol extract of Syzygium aqueum leaves had
antioxidant activity with IC50 value which was 20,24 ?g/mL and its xanthine
oxidase inhibitory activity was 47,22%, it was the highest amongst all plants.
Compound X has been isolated from methanol extract of Syzygium aqueum, that
was flavonoid, the purposed structure was 5,7,-dihydroxy,6,8-dimethyl flavanone.
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