ANTIDIABETIC ACTIVITY OF BLACK CUMIN (Nigella sativa Linn.) SEED POWDER AND ITS COMBINATION WITH GLICLAZIDE IN MICE INDUCE ALLOXAN

• Main Author: Andini Asad, Sheila
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45369
• Institution: Institut Teknologi Bandung

Background and objectives: In Indonesia, black cumin (JH) has been widely used for the treatment of degenerative diseases, one of them is diabetes mellitus. JH is generally used in seed powder form. Therefore, the aim of this study was to evaluate the effects of black cumin seeds to diabetes mellitus and also to evaluate the possibility of the mechanism of black cumin. Methods: Seed powder suspension form of JH was used in this study. This study used three methods. The first method was oral glucose tolerance test, and the second method was injection of 60 mg/kg alloxan intravenously at a single dose. The mice were divided into six groups: normal control, diabetic control, diabetic treated with 1300 mg/kg of JH (JH1), diabetic treated with 2000 mg/kg of JH (JH2), diabetic treated with 0,65 mg/kg of gliclazide, and diabetic treated with JH1-gliclazide. All of them was administered daily by intragastric gavage for 3 weeks. Blood glucose was determined every 7 days and the pancreas was observed by Gomori staining. The third method was to estimate insulin sensitivity. The mice were induced by intragasticly 0,01 ml/g of high fat emulsion for 2 weeks. The mice were divided into 4 groups; normal control, diabetic control, diabetic treated with JH1 (daily oral for 3 weeks) and diabetic treated with 195 mg/kg of metformin (daily oral for 3 weeks). Insulin sensitivity was determined by IVITT. Results: JH2 was better than JH1 in inhibiting the increasing of blood glucose 30 minutes after orally glucose administering. The combination of JH1-gliclazide could not normalize blood glucose level within 2 hours. JH1 and JH2 could reduce blood glucose levels significantly in mice-induced alloksan and had a better effect than gliclazide’s. The reduction of blood glucose level of the JH1-gliclazide combination was not better than the single use. JH1 did not increase the number of beta cells whereas JH2 was able to increase the number of beta cells 3.5 times greater than the control. In mice induced by high-fat emulsion, JH1 could improve insulin sensitivity. Conclusion: JH seed has an antidiabetic effect with some possible mechanisms, which are inhibiting the absorption of glucose, increasing the number of pancreatic beta cell, and improving insulin sensitivity. The combination of black cumin with gliclazide did not have a better effect than the single use of black cumin.