FORMULASI DAN UJI EFEK SEDIAAN EMULSI ORAL VIRGIN COCONUT OIL (VCO) TERHADAP GEJALA PUTUS OBAT PADA MODEL MENCIT KETERGANTUNGAN MORFIN

• Main Author: Rizkiya Azhary, Rahma
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45400
• Institution: Institut Teknologi Bandung

Morphine dependence make withdrawal syndrome when the users discontinued. The effects of withdrawal syndrome cause pain and inhibit the therapeutic process. A number of research reveal the involvement of neuroinflammation mechanism in withdrawal syndrome. Virgin Coconut Oil has a high free fatty acid that has effect on expression of inflammatory inhibition. However, the use of virgin coconut oil is less prefered on patients because of oil bad taste. Formulation of VCO required to increasing acceptance on patients without decrease the effect. The aims of the research are to prepare stable emulsion formulation of VCO and determine the effect of withdrawal on morphine dependent mice. Optimization stirring speed at 6000, 7000, and 8000 RPM was done with stability of emulsion within 7 days. Optimizations of emulsifier were performed on pH, viscosity, particle size, and high of sedimentation. Stability test would be evaluated for the best formula. The effect of withdrawal in morphine dependent mice would be tested in vivo. Six groups namely positive, negative, VCO, emulsion 0.26 mL/ 20 gr BW, emulsion 0.13 mL/ 20 gr BW, and emulsion based group were prepared. All groups except negative group were induced by morphine with in increasing doses from 8 mg/ kg BW to 45 mg/ kg BW and given intraperitoneally everyday in period of 5 days. In fifth day, all of morphine dependent groups received Naloxone HCl 3 mg/ kg BW subcutaneous. The others groups received VCO, emulsion, and emulsion base (p.o) 30 min prior to administration of Naloxone HCl. For the behavioral assessment, the animals were observed individually in a cylindrical chamber within 30 min after Naloxone injection. Stirring speed 7000 RPM showed high of sedimentation within 7 days. The best VCO emulsion were consist of 15%, 17.5%, 20 % gum acacia and 1% tragacanth as emulsifier. High of sedimentation and pH were evaluated in period of 7 days. Formulation A, B, C, and D were compared on pH value , viscosity, and high sedimentation within 28 days and resulting formula D was the best formulation. The Freeze Thaw test of Formula D showed stable result on pH value and particle size parameters. Unfortunately, centrifuge test showed separated phase after first hour. The effect of VCO group and Emulsion of 0.26 mL/ 20 gr BW group decreased withdrawal syndrome in morphine dependent mice for jumping test compared with positive group. The best formula consist of 44% w/v VCO, 20% w/v gum acacia, 0.05% w/v sucralose, 0.1% w/v potassium sorbate, 0.02% w/v Alpha-tocopherol, 2% w/v Pineapple essence, and 0.01% w/v D&C Yellow no.10. The VCO’s emulsion had effect on decrease withdrawal syndrome on morphine dependent mice.