FORMULASI DAN KARAKTERISASI NANOPARTIKEL KURKUMIN-PLA DENGAN METODE EMULSIFIKASI PENGUAPAN PELARUT

• Main Author: Lie Yanda, Yulia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45406
• Institution: Institut Teknologi Bandung

Curcumin is a polyphenolic compound derived from Curcuma longa Linn. (Zingiberaceae) that possesses diverse pharmacological effects including anti-inflammatory, antioxidant, antimicrobial, and anticarcinogenic activities. Although phase I clinical trials have shown curcumin as a safe drug even at high doses (12 g/day) in human, poor bioavaibility largely limited its pharmacological activity. This research aims to develop curcumin in polymeric nanoparticles by using PLA (Poly-(Lactic acid)). Nanoencapsulation in biodegradable polymers is a promising alternative to improve curcumin bioavaibility. The curcumin-PLA nanoparticle was formed via emulsification-solvent evaporation method. Various parameters to obtain sufficient property for medical application were studied, such as the type of solvent, surfactant concentration, sonication time and centrifugation speed. The influence of these parameters on the nanoparticle was evaluated initially like the particle size and polydispersity index. Further, drug loading (curcumin of 2-20%) was observed to obtain the optimum formula with highest encapsulation efficiency. Curcumin-loaded PLA nanoparticles were subsequently characterized for the particle size, polydispersity index, zeta potential, morphology, encapsulation efficiency, and in vitro drug release. Physical property of the pure curcumin, pure PLA, and the nanoparticles were also determined by differential scanning calorimetry (DSC), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). To obtain a smaller particle, dichloromethane as solvent, 0,03% vitamin E polyethylene glycol succinate (TPGS) as a surfactant, short sonication time of 4 min, and high centrifugation speed of 10500 rpm were selected. By emulsification-evaporation technique, the PLA with 5% curcumin formed nanoparticle containing of 89,42 ± 1,04 % curcumin, has size of 387,50 ± 58,60 nm and PI of 0,289 ± 0,047. The nanoparticle shows negatively charged of -1,12 mV. Based on DSC, XRD, and FTIR analysis, we suggest that there is a partial interaction between the drug and the polymer. After 48 hours, 54,43 % of the curcumin was released from curcumin loaded PLA nanoparticles. The optimum formula of curcumin loaded PLA nanoparticles was obtained with sufficient properties. This formula is promising to be an alternative for curcumin delivery system to improve its bioavaibility.