PENGARUH KONDISI HIPERGLISEMIA TERHADAP PEMBENTUKAN PLAK ATEROMA PADA TIKUS WISTAR JANTAN YANG DIINDUKSI HIPERKOLESTEROLEMIA

• Main Author: Yudha Ramadhan, Wieditha
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45468
• Institution: Institut Teknologi Bandung

Atherosclerosis is a chronic inflammatory disease caused by high cholesterol concentration in plasma, especially low-density lipoprotein (LDL) and inflammation in the arterial wall. The establishment of an animal model of atherosclerosis induced by high-cholesterol diet requires a long period of time. Hyperglycemia triggers an inflammatory response in the arterial walls, thus hyperglycemia involved in the atherosclerosis formation rate. The purpose of this study is to determine the effect of hyperglycemia on the atheroma plaque formation in hypercholesterolemiainduced male Wistar rats. Rats were divided into four groups, which are normal group, hyperglycemia group, hypercholesterolemia group and hyperglycemia –hypercholesterolemia group. Hyperglycemia was induced by administrating single dose of alloxan (100 mg/kg) intraperitoneally. Hypercholesterolemia was induced by feeding high cholesterol diet, cholesterol (100 mg/kg bw), cholic acid (0.2% amount of food) and propylthiouracil (12.5 mg/kg bw) via the oral route. Blood glucose levels measurement is done at the time before hyperglycemia induction, at five days after hyperglycemia induction (T0) and at the end of the experiment (T56). Measurement of total cholesterol, triglycerides, HDL and LDL is done on days 0, 14, 35, and 56 of hypercholesterolemia induction. The results of blood glucose measurement in rats induced by alloxan (276.00 –317.5 mg/dl) showed a significant difference compared to normal rats (82.71 – 84.42 mg/dl). Total cholesterol and LDL of hypercholesterolemia induced group (126.61 ± 37.09 mg/dl and 95.03 ± 27.97 mg/dl, respectively) and hyperglycemia –hypercholesterolemia induced group (143.89 ± 65.79 mg/dl and 87.62 ± 26.56 mg/dl, respectively) showed a significant difference compared to the normal group (73.29 ± 11.02 mg/dl and 42.64 ± 16.99 mg/dl, respectively) since the day 35 of induction. Triglyceride level of hyperglycemia and hypercholesterolemia induced group (206.00 ± 80.09 mg/dl) showed a significant difference compared to the normal group and diabetes induced group (76.85 ± 32.00 mg/dl) on day 14 of induction, however all induced group showed no significant difference compared to normal group on day 35 and 56. HDL levels of hyperglycemia all induced group showed no significant difference compared to the normal group during the experiment. Atherogenic index plasma of hyperglycemia induced group, hypercholesterolemia induced group and hyperglycemia –hypercholesterolemia induced group (0.423 ± 0.202, 0.356 ± 0.174 and 0.420 ± 0.094, respectively) showed a significant difference compared to the normal group (0.042 ± 0.231) on day 56 of induction. The aorta wall thickness of all induced group showed no significant difference compared to the normal group. The results showed that the hyperglycemia condition does not accelerate the atheroma plaques formation in male Wistar rats.