TELAAH FITOKIMIA RIMPANG KECOMBRANG HUTAN [ Etlingera hemisphaerica (Blume) R.M.Sm. ]

• Main Author: Puspita Puri, Gita
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45486
• Institution: Institut Teknologi Bandung

Zingiberaceae are distributed mostly in tropical and subtropical areas. The center of distribution is in South East Asia. The greatest concentration of genera and species is in the Malesian region (Indonesia, Malaysia, Singapore, Brunei, the Philippines and Papua New Guinea). It has been used for many years as spices and in traditional forms of medicine, one of the member is Etlingera hemisphaerica (Blume) R.M.Sm. (kecombrang hutan). The information and research about this species are still limited. The objectives of this research were to isolate, characterize, and identify marker compound from kecombrang hutan rhizome. Crude drug of kecombrang hutan rhizome was extracted by reflux method using three solvents with increasing polarity: n-hexane, ethyl acetate, and methanol. Ethyl acetate extract was fractionated using classic column chromatography with gradient elution system. Purification of fraction 31-60 was performed using TLC preparative to obtain isolate B. The purity of isolate B was tested by single-development system TLC using three mobile phases with different polarity and two-dimensional TLC. Isolate B was characterized using specific spray reagent, ultraviolet-visible spectrophotometry, twodimensional paper chromatography and infrared spectrometry. The phytochemical screening showed crude kecombrang hutan rhizome contained flavonoid, saponin, and steroid/triterpenoid. The maximum wavelength of isolate B were 280 and 359 nm. Infrared spectra showed peaks at 3424 cm -1 (-OH), 2923 cm -1 (-CH3), 1720 cm -1 (C=O), and 1427 cm -1 (C=C aromatic). Isolate B was proposed as flavonol aglycone.