UJI EFEK ANTIDEPRESAN DAN KETERGANTUNGAN ISOLAT ARILUS PALA (Myristica fragrans Houtt.) PADA MENCIT JANTAN GALUR SWISS-WEBSTER

• Main Author: Johanka, Septian
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45528
• Institution: Institut Teknologi Bandung

Depression is a kind of mental disorders characterized by sadness, anxiety and loss of interest or pleasure. It has been progressively increasing becoming a global public health concern in the world. Synthetic drug used to treat depression affects the central nervous system with a probable side effect of dependence, causing serious complication. Previous reseach has shown antidepressant effect of nutmeg extract from its shell. This research was aimed to further experiment active isolate of nutmeg that contributed most effect as an antidepressant and dependence drug to be used as an alternative herbal medicine to treat depression. Seven isolate was administered to seven groups, which are named A, B, C, D, E, F and G with dose of 10 mg/kg. Positive control group was administered amitriptyline and negative control group which was administered corn oil. All 9 groups were administered drug, corn oil or isolate for 14 days straight. At day 15, all admistrations of drugs was stopped for 24 hours. At day 1 and day 15, activity test which are open-field test and forced swim test was commenced and analyzed. Group A shown most active result from activity test was executed, its brains were dissected and used for ELISA analysis to calculate noradrenaline and dopamine level. In open-field test, group A showed significant difference (p< 0.05) in time spent in border on day 1 with value of 60.76±19.64 seconds compared to negative group with value of 89.31±12.01 seconds. In forced swim test, immobility time was measured and group A shown significant results (p< 0.05) on day 1 and day 15 with value 137.2±19.64 and 125.89±53.89 seconds respectively compared to negative group with value on day 1 and day 15 of 201.76±17.89 and 197.91±21.57 seconds respectively. Group A was chosen for ELISA analysis procedure. Group A shown noradrenaline value of 1.42±0.01 ng/mL sample shows significant difference (p< 0.05) compared to negative group. Group A also shows significant difference (p< 0.05) with value of 1.37±0.07 ng/mL. Experiment shows isolate A from nutmeg’s (Myristica fragrans Houtt.) middle shell with dose of 10 mg/kg mice which had been administered for 14 days straight indicates antidepressant effect and dependence with anxiolytic effect.