MODIFICATION OF CARBON PASTE ELECTRODE WITH Ag NANOPARTICLES and MOLECULARLY IMPRINTED POLY(GLUTAMIC ACID) FOR VOLTAMMETRIC DETERMINATION OF PHENOL

• Main Author: Sulistyorini, Tri
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/45666
• Institution: Institut Teknologi Bandung

Phenol is an aromatic derivative of hydrocarbons from coal which used as a base for making plastic bottles, cans of packaged food, antiseptic, and pesticides. The large amount of phenol in industrial fields cause phenol easily found in water. Analysis of phenol levels in the environment is very important because this compound is highly reactive to human body tissues if absorbed through contact with the skin, respiration, and digestion. Different method has been reported for analysis phenol such as voltammetry. The sensor used is carbon paste electrode modified Ag nanoparticles/CPE-nAg and modified poly(glutamic acid)/CPE-MIP. First, Ag nanoparticles synthesized with polyvynilpyrrolidone and NaBH4. The best composition ofCPE-nAg is 5:70:25 for Ag nanoparticles, graphite, and paraffin oil. In addition using Ag nanoparticles, carbon paste electrode also modified by poly(glutamic acid). Modifications were carried out through the electropolyrnerization process using cyclic voltammetry in a mixture of glutamic acid as a monomer and phenol as analyte solution dissolved in phosphate buffers 0, I M pH 9 solution. Phenol measurements were carried out in NaOH I M solution as a support ing electrolyte by square wave voltammetry (SWV) method in potential range of 0 V to 1,4 V, frequency of 10 Hz, and scan rate of 75 m V/s. Phenol measurements using carbon paste electrode modified Ag nanoparticles and molecularly imprinted poly(glutamic acid) could increase the anodic currrent response. The effect of comparison of Ag nanopartiles composition, comparison of monomer and analyte, number of cyclic electropolymerization, number of analytes removal cycles, and scan rate have been optimized in this study. Calibration curve ofthese electrodes showed a good linearity in the concentration range of 100 f.!M- 1000 f.!M with detection limit of 86 f.!M and 62 1-!M for CPE-nAg -and CPE MTP. CPE-nAg and CPE-MfP showed good reproducibility by producing %RSD of 4,97% and 4,69% {n=35). Determination of phenol levels in river, produces a pretty good measurement with recovery percentage of 96- 99% where this measurement is not much different from measurements using HPLC in confidence level of 95%.