FORMULATION OF CHITOSAN-ALOE VERA-PAPAIN MATRIX IN MICROPARTICLE AND GEL DOSAGE FORM FOR WOUND HEALING ACCELERATION
The aim of this study was to prepare chitosan matrix containing Aloe vera as growth factor and papain as debridement enzymatic and to evaluate its treatment efficacy on induced third degree dermal burns on rabbits. Chitosan microparticles were prepared by extrusion method. A.vera powder was dispe...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/45689 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The aim of this study was to prepare chitosan matrix containing Aloe vera as
growth factor and papain as debridement enzymatic and to evaluate its treatment
efficacy on induced third degree dermal burns on rabbits. Chitosan microparticles
were prepared by extrusion method. A.vera powder was dispersed in chitosan
solution in lactic acid and extruded into 10% sodium tripolphosphate solution at
pH 3 under continuous stirring. Papain was adsorbed onto chitosan - A.vera
microparticles. Encapsulation efficiency of A.vera was determined by measuring
malic acid concentration in microparticles by using HPLC instrument, while
adsorption capacity of papain was determined by measuring total protein using
Bradford reagent. A.vera encapsulated and papain adsorbed were 84.83±0.12%
and 10.11±0.38% respectively. Chitosan film was prepared by casting of chitosan
solution in lactic acid with glycerin as plasticizer. The best formula of chitosan
film was resulted from 2% of chitosan dissolved in lactic acid 2% and 30% (w/w)
glycerin. The healing of burn wounded rabbits was analyzed by application of a
chitosan composite consisting of chitosan - A.vera - papain microparticles covered
with chitosan film (MCAPF). Wound treated by chitosan (MCF), chitosan -
A.vera (MCAF), chitosan gel and non-treated wound were analyzed as
comparison. Macroscopically, the MCAPF matrix showed the largest percentage
of wound contraction. However, there was no statistical difference of wound
contraction percentage between treatments (p<0.05). While, wound treated by
chitosan film and chitosan - A.vera - papain gel (GCAPF) showed the largest
percentage of wound contraction until the 7th
day of treatment. Encouragingly, the
biopsy samples taken at 21 days after treatment of the MCAF and MCAPF
matrices showed minimum number of inflammatory cells. This was implied the
activity of A.vera as anti-inflammatory. The papain - A.vera loaded chitosan
matrix is potential to be used for the treatment of third degree dermal burns.
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