PREPARATION OF PVA NANOFIBER FOR PROTEIN DELIVERY BY ELECTROSPINNING METHOD AND ITS MODIFICATION FOR pH RESPONSIVE RELEASE

• Main Author: Risdian, Chandra
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45788
• Institution: Institut Teknologi Bandung

Electrospinning is a process to form fibers by utilizing electrostatic forces. Recently, nanofibers have been applied in wide variety fields including filtration, protective clothing, scaffold for tissue engineering, and drug delivery e.g therapeutic protein. Protein delivery by oral route has many obstacles such as low permeability, lack of lipophilicity, rapid inactivation and enzymatic degradation in the gastrointestinal tract. The aim of this study was to prepare PVA nanofiber for protein delivery system by electrospinning method and its modification with pH responsive polymer for enteric relesae. Bovine serum albumin (BSA) was used as a model of protein, while Eudragit L-100 was used as a pH responsive polymer. PVA nanofiber was prepared by electrospinning method. PVA nanofiber was prepared with various polymer concentrations, voltages, flow rates, needlecollector lengths and addition of surfactant (Triton X-100). PVA/BSA (20:1) nanofiber was produced with various voltages only. Characterization of nanofiber was evaluated by optical microscopy, SEM, EDS, FTIR, DSC, and XRD. Modification of PVA/BSA nanofiber with Eudragit L-100 was conducted by dip coating method. Smooth and uniform PVA nanofiber was produced with concentration of PVA 10% (w/v), voltage 15 kV, flow rate 3 µL/minute, needlecollector length of 12 cm, and addition of Triton X-100 (0,05% w/v). Smooth and uniform PVA/BSA (20:1) nanofiber was obtained by voltage 12 kV, flow rate 3 µL/minute, needle-collector length of 12 cm, and 0,05% Triton X-100 (w/v). Diameter size of PVA nanofiber was 294 ± 45 nm while diameter size of PVA/BSA (20:1) nanofiber was 319 ± 50 nm. An in vitro evaluation for protein relase was studied using HCl buffer pH 1.2 and phosphate buffer pH 6.8. Modification of PVA/BSA nanofiber with Eudragit L-100 delayed the release of BSA in HCl pH 1.2 for 2 hours. In contrast, BSA from PVA/BSA nanofiber coated with Eudragit L-100 was released in phosphate buffer pH 6.8 for 22 hours. This result suggests that Eudragit L-100-coated PVA nanofiber containing BSA shows enteric release of BSA.